domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, reverse phase protein lysate microarray, other
citations: 6
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, reverse phase protein lysate microarray, other
citations: 6
Western blot using Rockland's affinity purified anti-Pdcd4 antibody shows detection of a band ~52 kDa in size corresponding to Pdcd4 (arrowhead). Lane 1 contains recombinant Pdcd4. Lane 2 contains 293 HEK cells treated with TPA and MG132. The anti-Pdcd4 antibody was used at a 1: 5,000 dilution. Personal Communication. M Young & A Jansen, NCI, Bethesda, MD.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (9G6)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
citations: 2
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
citations: 2
mouse monoclonal (9G6)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry
citations: 1
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry
citations: 1
Rockland Antibody 200-301-964 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WA
quantity: 100 µg
price:
to the supplier
mouse monoclonal (9G6)
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, ELISA, immunoprecipitation
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, ELISA, immunoprecipitation
ELISA of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Antigen: BSA conjugates of Pdcd4 phospho S457 and Pdcd4 non-phospho S457. Coating amount: 0.1 µg per well. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 5 µg/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL Pdcd4 phospho S457 Biotin Conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-0100)
quantity: 50 µg
price:
to the supplier