Collagen Type I Antibody
Rockland Immunochemicals
catalog: 600-401-103-0.1
domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, dot blot
citations: 136

Western blot analysis is shown using Rockland's Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading.  An equal amount of the whole cell protein (100 µg) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes.  Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2–2 µg/10 ml in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk.  Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 µg/10 ml.  Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences).  Other detection systems will yield similar results. See Hazra et al. (2004) for additional details.

Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.
quantity: 100
price: 512.00 USD
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Collagen Type I Antibody
Rockland Immunochemicals
catalog: 600-401-103-0.5
domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
citations: 120

Rockland's Affinity Purified anti-Collagen I antibody was used at a 1:100 dilution to detect distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. The antibody was reacted with antibody for 4 hours at room temperature followed by the addition of secondary antibody and substrate reaction. Tissue was formalin-fixed and paraffin embedded. No antigen retrieval was performed.
quantity: 500
price: https://www.rockland.com/search/?searchString=600-401-103-0.5
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Collagen Type I Antibody Biotin Conjugated
Rockland Immunochemicals
catalog: 600-406-103
domestic rabbit polyclonal
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, flow cytometry, dot blot
citations: 13

Western Blot of Rabbit anti-Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 µg per lane. Protein staining shown below each blot depicts equal protein loading. Primary antibody: anti-Collagen I antibody at 0.2–2 µg/10 ml for overnight at 4°C. Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 µg/10 ml for overnight at 4°C. Block: TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I. Other band(s): none.
quantity: 100
price: 522.00 USD
to the supplier
Collagen I alpha 1 propeptide Antibody
Rockland Immunochemicals
catalog: 600-401-D19
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 2

Immunofluorescence Microscopy of Rabbit Anti-Collagen I -alpha 1-propeptide antibody. Tissue: fibrotic mouse lung tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Collagen I antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen I is an extracellular matrix protein. Staining: Collagen I molecules (red) with bis-benzimide (blue) nuclear counterstain.
quantity: 100 µL
price: 366.00 USD
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Collagen Type I Antibody Fluorescein Conjugated
Rockland Immunochemicals
catalog: 600-402-103
domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
citations: 2
quantity: 50
price: 469.00 USD
to the supplier
Collagen I alpha 1 telopeptide Antibody
Rockland Immunochemicals
catalog: 600-401-D20
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
citations: 1

Western Blot of Anti-Collagen 1, alpha 1 telopeptide (Rabbit) Antibody. Lane 1: rat lung lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Collagen 1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~ 140kDa/~140kDa for collagen protein. Other band(s): none.
quantity: 100
price: https://www.rockland.com/search/?searchString=600-401-D20
to the supplier
Collagen Type I Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 600-403-103
domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
quantity: 50 µg
price:
to the supplier