Rockland Immunochemicals H4-16 antibody

Histone H4 K20me1 Antibody
Rockland Immunochemicals
catalog: 600-401-J01

domestic rabbit polyclonal
reactivity: human, mouse, roundworm
application: western blot, immunohistochemistry, chromatin immunoprecipitation
citations: 1

Immunofluorescence of Rabbit Anti-Histone H4 [Monomethyl Lys20] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [Monomethyl Lys20] antibody at a 1:500 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [Monomethyl Lys20] is nuclear and chromosomal. Staining: Histone H4 [Monomethyl Lys20] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).

Western Blot of Rabbit Anti-Histone H4 [Monomethyl Lys20] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H4 [Monomethyl Lys20] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.

Chromatin Immunoprecipitation Rabbit Anti-Histone H4 [Monomethyl Lys20] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Histone H4K20me1 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H4 pS1 Antibody
Rockland Immunochemicals
catalog: 600-401-I96

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Immunofluorescence of Rabbit Anti-Histone H4 [p Ser1] Antibody. Tissue: Nonmitotic and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [p Ser1] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [p Ser1] is nuclear. Staining: Histone H4 [p Ser1] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).

Western Blot of Rabbit Anti-Histone H4 [p Ser1] Antibody. Lane 1: HeLa histone prep. Load: 30 µg per lane. Primary antibody: Histone H4 [p Ser1] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.

Immunofluorescence of Rabbit Anti-Histone H4 [p Ser1] Antibody. Tissue: Nonmitotic and telophase HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H4 [p Ser1] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4 [p Ser1] is nuclear. Staining: Histone H4 [p Ser1] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 594 (red).
quantity: 50 µg
price:
to the supplier

Histone H4 R3me1 Antibody
Rockland Immunochemicals
catalog: 600-401-I97

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H4 [Monomethyl Arg3] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [Monomethyl Arg3] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.

Western Blot of Rabbit Anti-Histone H4 [Monomethyl Arg3] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [Monomethyl Arg3] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.
quantity: 50 µg
price:
to the supplier

Histone H4 K5ac Antibody
Rockland Immunochemicals
catalog: 600-401-I98

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H4 [ac Lys5] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H4 [ac Lys5] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Rabbit Anti-Histone H4 [ac Lys5] Antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2 ug of Histone H4 K5ac was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
quantity: 50 µg
price:
to the supplier

Histone H4 K8ac Antibody
Rockland Immunochemicals
catalog: 600-401-I99

domestic rabbit polyclonal
reactivity: human, mouse, roundworm
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H4 [ac Lys8] Antibody. Lane 1: HeLa histone prep. Lane 2: 3T3 histone prep. Lane 3: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H4 [ac Lys8] at 0.2 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~11 kDa. Other band(s): None.

Dot Blot of Rabbit Histone H4 [ac Lys8] Antibody. Lane 1: K8 Ac. Lane 2: K8 unmodified. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H4 [ac Lys8] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Chromatin Immunoprecipitation Rabbit Anti-Histone H4 [ac Lys8] Antibody. Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of Histone H4 K8ac and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H4 K16ac Antibody
Rockland Immunochemicals
catalog: 600-401-J00

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Western Blot of Rabbit Anti-Histone H4 [ac Lys16] Antibody. Lane 1: HeLa histone prep. Lane 2: NIH-3T3 histone prep lysate. Load: 30 µg per lane. Primary antibody: Histone H4 [ac Lys16] at 0.2 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.

Dot Blot of Rabbit Histone H4 [ac Lys16] Antibody. Lane 1: K16Ac. Lane 2: K16. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H4 [ac Lys16] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
quantity: 50 µg
price:
to the supplier

Histone H4 K20me2 Antibody
Rockland Immunochemicals
catalog: 600-401-J02

domestic rabbit polyclonal
reactivity: human, roundworm
application: western blot, immunohistochemistry, chromatin immunoprecipitation

Chromatin Immunoprecipitation of Rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H4 K20me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.

Western Blot of Rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H4 [Dimethyl Lys20] at 1:2000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~13 kDa. Other band(s): None.

Chromatin Immunoprecipitation of Rabbit Anti-Histone H4 [Dimethyl Lys20] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Histone H4 K20me2 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
quantity: 50 µg
price:
to the supplier

Histone H4 Antibody
Rockland Immunochemicals
catalog: 100-401-BR9

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

quantity: 100 µL
price:
to the supplier

Histone H4 K12-Ac Antibody
Rockland Immunochemicals
catalog: 600-401-P06

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

quantity: 50 µg
price:
to the supplier

Histone H4 pan Antibody
Rockland Immunochemicals
catalog: 600-401-X46

domestic rabbit polyclonal
reactivity: human
application: ELISA, chromatin immunoprecipitation

quantity: 50 µg
price:
to the supplier