mouse monoclonal (10H11.E12)
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, ELISA, immunocytochemistry, flow cytometry, western blot knockout validation
citations: 122
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, ELISA, immunocytochemistry, flow cytometry, western blot knockout validation
citations: 122
Anti ATM Mab with human derived HEK293 cells treated with doxorubicin using Rockland's Protein A Purified Mab anti-ATM Protein Kinase pS1981(clone 10H11.E12). A 370 kDa band corresponding to phosphorylated ATM is detected (arrowhead, lane 2). The lysate was prepared with HALT phosphatase inhibitor (Pierce). Pre-incubation of peptide with 50 µg of immunizing phospho peptide negates specific staining (lane 1). Approximately 30 µg of lysate was added to each lane of an SDS-PAGE gel under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:500 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Mouse IgG [H&L] (code 610-132-121) for 40 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results
quantity: 100
price: 596.00 USD
to the supplier
mouse monoclonal (7C10D8)
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 11
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 11
Anti ATM monoclonal Antibody was used to show constitutive activation of the ATM pathway in human urinary bladder cancer. Anti-ATM Protein Kinase pS1981 (clone # 7C10D8 ) was used to stain normal uroepithelium, early superficial lesions (Ta), and earliest invasive (T1) primary carcinomas. ATM protein is ubiquitously expressed, but Ser 1981-phosphorylated ATM (pS-ATM) is detectable only in tumor tissues. See Bartkova et al. for additional details. Reprinted by permission from Nature (Cell Cycle 4;(6), 2005) Macmillan Publishers Ltd.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry
Polyclonal rabbit anti ATM antibody detects ATM in HeLa Nuclear lysates run on 4-8% gel and transferred for 1 hr at 100V to 0.45 um nitrocelllose Membrane was Blocked in 5% Blotto 1 Hour 4oC. HRP Goat anti Rabbit 611-103-122 Lot#21231 was used for detection 1:20,000 in MB-070 1 Hour 4oC
quantity: 100 µg
price:
to the supplier
mouse monoclonal (10H11.E12)
reactivity: human, mouse, rat
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse, rat
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
Rockland's anti-ATM pS1981 mouse monoclonal antibody (Catalog # 200-301-400) detects ATM phosphorylated on Ser 1981 by Indirect immunofluorescence microscopy. Shown are hTCEpi cells (courtesy of Dr. Danielle Robertson) infected with HSV-1 at MOI 5.0 and fixed at 8 hpi with 3% paraformaldehyde/2% sucrose for 10 min. After rinsing, cells were permeabilized with 0.5% Triton X-100 for 5 min, blocked with 3% BSA for 30 min, and stained with Rockland's primary anti-ATM pS1981 antibody overnight at 5 µg/mL (1:200). Secondary staining was performed with Alexa Fluor 594 anti-mouse antibody. Images were taken with Olympus AX70 compound epifluorescence microscope equipped with Spot RT Slider camera. Experiment was performed by Oleg Alekseev in the laboratory of Dr. Jane Azizkhan-Clifford at Drexel University College of Medicine.
quantity: 100 µg
price:
to the supplier
mouse monoclonal (10H11.E12)
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse, rat
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
Anti ATM Mab with human derived HEK293 cells treated with doxorubicin using Rockland's Protein A Purified Mab anti-ATM Protein Kinase pS1981(clone 10H11.E12). A 370 kDa band corresponding to phosphorylated ATM is detected (arrowhead, lane 2). The lysate was prepared with HALT phosphatase inhibitor (Pierce). Pre-incubation of peptide with 50 µg of immunizing phospho peptide negates specific staining (lane 1). Approximately 30 µg of lysate was added to each lane of an SDS-PAGE gel under non-reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:500 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Mouse IgG [H&L] (code 610-132-121) for 40 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
quantity: 100 µg
price:
to the supplier