domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
Rockland's Anti-IKKa antibody was diluted 1:500 to detect IKKa in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
quantity: 100 µL
price:
to the supplier
mouse monoclonal (14A231)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry
Immunohistochemistry of mouse Anti-IKK alpha antibody. Tissue: human cervix tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: IKK alpha at 5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: Shuttles between the cytoplasm and the nucleus. Staining: IKK alpha antibody is stained using a DAB chromogen and Hematoxylin counterstain.
Western Blot of Mouse Anti-IKK alpha antibody. Lane 1: Daudi cell lysate. Load: 30 µg per lane. Primary antibody: IKK alpha antibody at 1 µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 84.6 kDa for IKKa. Other band(s): none.
Flow Cytometry of Mouse Anti-IKK alpha antibody. Cells: HEK293 cells. Stimulation: none. Primary Antibody: IKKa antibody at 0.5 µg/mL (red) and isotype control (green). Secondary Antibody: anti-mouse IgG1 Phycoerythrin conjugated secondary antibody.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA