product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Sheep Red Blood Cell RBC Antibody
catalog :
113-4139
quantity :
2
price :
239.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
mouse
application :
immunocytochemistry
more info or order :
citations: 3
| Published Application/Species/Sample/Dilution | Reference |
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product information
Catalog Number :
113-4139
Name :
Anti-SHEEP Red Blood Cell (RBC) (RABBIT) Antibody - 113-4139
Display Name :
Sheep Red Blood Cell RBC Antibody
Application Note :
Suitable for agglutination of cells on titer plates. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Blood Product Sterility :
Non-sterile
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
85 mg/mL
Concentration Definition :
by Refractometry
Conjugation :
(None)
Size :
2
Default Unit :
mL
Expiration :
Expiration date is one (1) year from date of opening.
Format :
Antiserum
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Sheep washed pooled Red Blood Cells (RBC)
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This product was prepared from polyspecific antiserum by delipidation and defibrination.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
2.0 mL
Tested Applications :
Agglutination
Suggested Applications :
Cellular Assay, Functional Assay
Storage :
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit Anti-sheep RBC antibody, Red Blood Cell Antibody, Antibody for hemagglutination, rabbit anti-sheep red blood cell, rabbit antibody to sheep Red Blood Cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent, anti-erythrocyte, anti-erythrocyte antibody
Target Species :
Sheep
Background :
Anti-SHEEP Red Blood Cell Antibody may be used in hemagglutination assays. Haemagglutination assay or HA is a method of quantification for viruses or bacteria by hemagglutination. Some viral families and many bacteria have envelope or surface proteins which are able to agglutinate (stick to) human or animal red blood cells (RBC) and bind to N-acetylneuraminic acid. As each of the agglutinating molecule attaches to multiple RBCs, a lattice-structure will form. Normally, a virus dilution (e.g. 2-fold from 1:4 to 1:4096) will be applied to an RBC dilution (e.g. 0.1% to 0.7% in steps of 0.2%) for approx. 30 min, often at 4° C, otherwise viruses with neuraminidase activity will detach the virus from the RBCs. Then the lattice forming parts will be counted and the titer calculated. The titer of a hemagglutination assay is determined by the last viable "lattice" structure found. This is because it is at the point where, if diluted anymore, the amount of Virus particles will be less than that of the RBCs and thus not be able to agglutinate them together. Anti-SHEEP Red Blood Cell Antibody is used to sensitize erythrocytes and quantitate agglutination.
Blood Product Type :
Red Blood Cells
Immunogen Type :
Other
Low Endotoxin :
No
Sample Size :
No
Other :
AGGLUTINATION TITER 1:32 to 1:64
Category :
Blood Products
Conjugation Name :
Unconjugated
2023 Price :
239.00 USD
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.
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