product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
IL-1 Receptor Type I Antibody
catalog :
109-401-304-0100
quantity :
100 µL
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
109-401-304-0100
Name :
Anti-Human IL-1 Receptor Type I (IL-1RI) (RABBIT) Antibody - 109-401-304-0100
Display Name :
IL-1 Receptor Type I Antibody
Application Note :
This antiserum against anti-Human IL-1 Receptor Type I (IL-1RI) has been tested for use in ELISA,  radioimmunoassays and immunoprecipitation.  Reactivity in other immunoassays is unknown. This product has been assayed for the ability to immunoprecipitate antigen.  A dilution range of 1:400 to 1:800 is suggested for this immunoassay.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by ELISA against soluble (extracellular) IL-1RI using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2000 is suggested for this product.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay.
Buffer :
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
84 mg/mL
Concentration Definition :
by Refractometry
Conjugation :
(None)
Size :
100 µL
Default Unit :
ul
ELISA Dilution :
User Optimized
Flow Cytometry Dilution :
User Optimized
Immunohistochemistry Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is one (1) year from date of opening.
Format :
Antiserum
Gene Name :
IL1R1
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
IL-1 Receptor Type I Antibody was prepared by repeated immunizations with recombinant human IL-1R type I.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This antiserum has been heated to 56°C for 30 minutes.  The antiserum is directed against the extracellular domain of the human IL-1R type I, but precipitates the intact receptor expressed on human cells.   In general, this antibody also detects primate IL-1RI.  The antibody does not recognize human IL-1, IL-1Ra or IL-1R type II.  In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type I (also called soluble IL-1R type I or ''decoy'' receptor) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.
Species Reactivity :
human, primate
Storage :
Store IL-1 Receptor Type I Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
IL1RT1 antibody, Interleukin 1 receptor alpha type 1 antibody, Interleukin 1 receptor antagonist protein (IRAP) antibody, Antigen CD121a antibody, CD121A antibody, CD121a antigen antibody, D2S1473 antibody, IL 1 antibody, rabbit anti-IL-1 Receptor Type I Antibody, rabbit anti-interleukin-1 Receptor Type I Antibody
Target Species :
Human
Background :
IL-1 Receptor Type I is a cytokine receptor which binds interleukin 1. Anti-IL-1 Receptor Type I antibody is ideal for researchers interested in Immunology, Signal Transduction and Cancer.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Neutralization Dilution :
User Optimized
Sample Size :
No
Application Text :
ELISA,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
P14778
NCBI :
P14778.1
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.