domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
citations: 10
reactivity: human
application: western blot, ELISA, immunohistochemistry
citations: 10
Western Blot of Rabbit Anti-Alpha Tubulin Antibody. Lane 1: whole cell lysates from mouse brain. Lane 2: rat brain. Lane 3: A431 cells. Lane 4: Jurkat cells. Lane 5: HeLa cells. Load: 35 µg per lane. Primary antibody: Alpha Tubulin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa corresponding to alpha tubulin (arrowhead). Other band(s): none.
quantity: 200 µg
price:
to the supplier
mouse monoclonal (17H11.F10)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 6
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
citations: 6
Rockland's anti-a-tubulin monoclonal antibody was used at a 2.5 µg/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Vasiliki Demas, LifeSpanBiosciences, Seattle, WA.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry