rat monoclonal (5.C3.1)
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation
citations: 2
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation
citations: 2
Immunofluorescence microscopy using Rockland's Protein G Purified Rat Monoclonal Anti-p19Arf antibody to detect endogenous mouse p19Arf protein present in sections of testis from a wild type mouse at stage P11, probed with 5-C3-1 MAb (green). The section was also incubated with DAPI, to show positions of nuclei (blue). A similar section from the testis of an Arf -/- mutant mouse does not stain with this MAb (data not shown). Tissue was perfused with 4% paraformaldehyde in PBS. Slides were placed in a jar containing citrate buffer at pH 6.0 at 96° C for 30 min followed by blocking in 10% normal goat serum in 0.1% Triton X-100-PBS for 30 min. Slides were incubated with 5 ug/ml antibody at 4° C overnight followed by additional of fluorochrome conjugated secondary antibody. See Bertwistle et al. 2004 for additional details.
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
Rockland's anti-p16 antibody was diluted 1:500 to detect p16 in human prostate tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
quantity: 100 µL
price:
to the supplier
rat monoclonal (12.A1.1)
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation
reactivity: human, mouse
application: western blot, ELISA, immunoprecipitation
Western blot analysis is shown using Rockland's Protein A Purified Rat Monoclonal Anti-p19Arf antibody to detect mouse p19Arf protein present in p19Arf transformed NIH-3T3 cells. Approximately 30 ?g of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer. The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by washing. A 1:20,000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemical's SuperSignal substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kDa corresponding to the expected molecular weight for human PMS2 protein. Other detection systems will yield similar results. See Bertwistle et al. 2004 for details).
quantity: 100 µg
price:
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry