domestic goat polyclonal
reactivity:
application: western blot, ELISA, immunoprecipitation
reactivity:
application: western blot, ELISA, immunoprecipitation
Rockland Goat anti Glutamine Synthetase antibody (200-101-238 lot 8182) was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.
quantity: 2 mL
price:
to the supplier
domestic goat polyclonal
reactivity:
application: western blot, ELISA, immunoprecipitation
reactivity:
application: western blot, ELISA, immunoprecipitation
Western Blot of Goat anti-Glutamine Synthetase antibody. Lane 1: Glutamine Synthetase Reduced. Lane 2: Glutamine Synthetase Non-Reduced. Load: ~1ug of protein per lane. Primary antibody: Glutamine Synthetase antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 53.3 kDa, 56 kDa for Glutamine Synthetase. Other band(s): Glutamine Synthetase splice variants and isoforms.
quantity: 100 µg
price:
to the supplier
domestic goat polyclonal
reactivity:
conjugate: biotin
application: western blot, ELISA, immunoprecipitation
reactivity:
conjugate: biotin
application: western blot, ELISA, immunoprecipitation
Rockland Goat anti Glutamine Synthetase antibody (200-101-238 lot 8182) was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.
quantity: 100 µg
price:
to the supplier
Glutamine Synthetase Antibody Peroxidase Conjugated
Rockland Immunochemicals
catalog: 200-103-238-0100
Rockland Immunochemicals
catalog: 200-103-238-0100
domestic goat polyclonal
reactivity:
conjugate: HRP
application: western blot, ELISA, immunoprecipitation
reactivity:
conjugate: HRP
application: western blot, ELISA, immunoprecipitation
Rockland Goat anti Glutamine Synthetase antibody (200-101-238 lot 8182) was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.
quantity: 100 µg
price:
to the supplier