Thymidylate Synthase Antibody | Rockland Immunochemicals 100-601-199 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Thymidylate Synthase Antibody

catalog :

100-601-199

quantity :

2 mL

clonality :

polyclonal

host :

domestic sheep

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1	Terranova Barberio M, Roca M, Zotti A, Leone A, Bruzzese F, Vitagliano C, et al. Valproic acid potentiates the anticancer activity of capecitabine in vitro and in vivo in breast cancer models via induction of thymidine phosphorylase expression. Oncotarget. 2016;7:7715-31 pubmed publisher

product information

Catalog Number :

100-601-199

Name :

Anti-THYMIDYLATE SYNTHASE (SHEEP) Antibody - 100-601-199

Display Name :

Thymidylate Synthase Antibody

Application Note :

This antiserum against Human Thymidylate Synthase (hTS) has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy, immunohistochemistry and immunoblotting. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes the 36,000 MW hTS. Reactivity in other immunoassays is unknown.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

85 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

2 mL

Default Unit :

ELISA Dilution :

1:10,000 - 1:50,000

Immunohistochemistry Dilution :

1:100

Western Blot Dilution :

1:500- 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

TYMS

Host Animal :

Sheep

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This whole sheep serum was prepared by repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This antiserum is directed against human Thymidylate Synthase and is useful in determining its presence in various assays. Because inhibition of Thymidylate Synthase prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

2.0 mL

Species Reactivity :

human

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

sheep anti-Thymidylate Synthase Antibody, HsT422 antibody, MGC88736 antibody, TMS antibody, TS antibody, TSase antibody, TYMS antibody, TYMS protein antibody

Target Species :

Human

Background :

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript rTSalpha vary inversely when cell-growth progresses from late-log to plateau phase. Diseases associated with Thymidylate synthase include Rectal Neoplasm and Dihydropyrimidine Dehydrogenase Deficiency. Anti-Thymidylate synthase is useful for researchers interested in Circadian Rhythm, Metabolism and cell cycle research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P04818

NCBI :

P04818.3

Primary Image Name :

Anti-Thymidylate Synthase Antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage