product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Anti-Urease Antibody
catalog :
100-4182
quantity :
2 mL
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunoprecipitation
more info or order :
Rockland Immunochemicals product webpage
citations: 1
Reference
Myrach T, Zhu A, Witte C. The assembly of the plant urease activation complex and the essential role of the urease accessory protein G (UreG) in delivery of nickel to urease. J Biol Chem. 2017;292:14556-14565 pubmed publisher
product information
Catalog Number :
100-4182
Name :
Anti-UREASE (Jack Bean) (RABBIT) Antibody - 100-4182
Display Name :
Anti-Urease Antibody
Application Note :
Anti-Urease Antibody has been assayed against 1.0 ug of Urease [Jack Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for Anti-Urease Antibody.
Buffer :
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
90 mg/mL
Concentration Definition :
by Refractometry
Conjugation :
(None)
Size :
2 mL
Default Unit :
mL
ELISA Dilution :
1:5,000 - 1:20,000
Immunoprecipitation Dilution :
1:100
Western Blot Dilution :
1:500 - 1:5,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
Antiserum
Gene Name :
UREA_CANEN
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Urease [Jack Bean]
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-Urease (Jack Bean) (Rabbit) Antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Urease [Jack Bean].  Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
2.0 mL
Species Reactivity :
Jack Bean (Canavalia ensiformis)
Storage :
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
rabbit anti-Anti-Urease Antibody, Urease, Urea amidohydrolase
Target Species :
Jack Bean
Background :
Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein. Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research.
Immunogen Type :
Native Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
P07374
NCBI :
AAA83831.1
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.