Myeloperoxidase Antibody | Rockland Immunochemicals 100-4181 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Myeloperoxidase Antibody

catalog :

100-4181

quantity :

2 mL

price :

1049 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

100-4181

Name :

Anti-MYELOPEROXIDASE (Human White Blood Cell) (RABBIT) Antibody - 100-4181

Display Name :

Myeloperoxidase Antibody

Application Note :

This antibody is suitable for western blotting, ELISA and immunoprecipitation. The antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combination (68 kDa and 106 kDa). Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods. Anti-Human Myeloperoxidase may react with MPO from other sources. Anti-Human Myeloperoxidase detects neutrophilic granulocytes and monocytes in blood and precursors of granulocytes in the bone marrow. This antibody may also detect myeloid leukemias of the bone marrow as well as other sites.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

75 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

2 mL

Default Unit :

ELISA Dilution :

1:200,000

Immunoprecipitation Dilution :

1:100

Western Blot Dilution :

1:5,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

MPO

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Myeloperoxidase [Human Leukocytes]

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Myeloperoxidase [Human Leukocytes]. Cross reactivity against Myeloperoxidase from other tissues and species may occur but have not been specifically determined.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

2.0 mL

Species Reactivity :

human

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Myeloperoxidase Antibody, Myeloperoxidase, MPO

Target Species :

Human

Background :

Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H 2 O 2 ). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.

Immunogen Type :

Native Protein

Low Endotoxin :

Sample Size :

Application Text :

ELISA,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

1049 USD

UniProt :

P05164

NCBI :

P05164.1

Primary Image Name :

Anti-Myeloperoxidase (Human White Blood Cell) Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage