NFkB p65 Antibody | Rockland Immunochemicals 100-4165 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

NFkB p65 Antibody

catalog :

100-4165

quantity :

100 µL

price :

369 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, chromatin immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; rat; fig 4	Zhu X, Wang K, Zhang K, Tan X, Wu Z, Sun S, et al. Tetramethylpyrazine Protects Retinal Capillary Endothelial Cells (TR-iBRB2) against IL-1Î²-Induced Nitrative/Oxidative Stress. Int J Mol Sci. 2015;16:21775-90 pubmed publisher

product information

Catalog Number :

100-4165

Name :

Anti-NFKB p65 (Rel A) (RABBIT) Antibody - 100-4165

Display Name :

NFkB p65 Antibody

Application Note :

Anti-NFkB p65 (Rel A) is suitable for the detection by immunoblot of human and mouse NFkB p65 (Rel A), immunohistochemistry, and ELISA. This product was also tested in a gel supershift assay and ChIP and found to be reactive against all p65 (Rel A) containing human, mouse or rat NFkB complexes using 0.5 to 1.0 µl per assay.

Clonality :

Polyclonal

Concentration Value :

80 mg/ml

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:5,000

ChIP Dilution :

1 μl/IP

Gel Shift Dilution :

05 µL - 1.0 µL

Immunohistochemistry Dilution :

1:400

Western Blot Dilution :

1:2,000 - 1:5,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

RELA

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

NFkB p65 (Rel A) was prepared from monospecific antiserum by delipidation and defibrination. Anti-NFkB p65 (Rel A) may react non-specifically with other proteins. Control peptide (code #100-4165p) will compete only with the specific reaction of antiserum with the NFkB p65 (Rel A) subunit.

Species Reactivity :

human, mouse, rat

Storage :

Store NF-kappaB antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit Anti-NFKB p65 antibody, rabbit Anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit

Target Species :

Human

Background :

Anti-NFkB p65 Antibody recognizes NFKB p65 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. IkappaBalpha-binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.

Specific Reference :

Gregory et al. (2004) Efficient replication by herpes simplex virus type 1 involves activation of the IkappaB kinase-IkappaB-p65 pathway. J Virol. 2004 Dec;78(24):13582-90. PMID: 15564469 - WB. Poser et al. (2003) Upregulation of HMG1 Leads to Melanoma Inhibitory Activity Expression in Malignant Melanoma Cells and Contributes to Their Malignancy Phenotype. PMID: 12665595 - IP/Co-IP. Jobin et al. (1999) Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity. PMID: 10477620. Cao et al. (2006) NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages. J Bio Chem. 2006 Sep 8;281(36):26041-26050. Lou et al. (2007) Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms. J Bio Chem. Oct 5;282(40):29470-29481. Yoon et al. (2008) Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype. J. Neuro. Apr 2;28(14):3738-3746. Begley et al. (2008) CXCL5 promotes prostate cancer progression. Neoplasia. Mar;10(3):244-254. PMID: 18320069-WB. Begley et al. (2005) CXCL12 overexpression and secretion by aging fibroblasts enhance human prostate epithelial proliferation in vitro. PMID: 16300481 - WB. Limpert et al. (2010) Axonal Neuregulin 1 Type III Activates NF-kappa B in Schwann Cells during Myelin Formation. J Bio Chem 285 (22): 16614-16622. Cheng et al. (2003) Cyclin E and Bcl-xL cooperatively induce cell cycle progression in c-Rel-/- B cells. Oncogene. PMID: 14627988 - WB. Fan et al. (2004) Divergent C-terminal transactivation domains of Rel/NF-kappa B proteins are critical determinants of their oncogenic potential in lymphocytes. PMID: 14647412.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Chip,Gel Shift,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

369 USD

UniProt :

Q04206

NCBI :

223468676

Primary Image Name :

NFkB p65 (Rel A) antibody – IHC

more info or order :

Rockland Immunochemicals product webpage