product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Alcohol Dehydrogenase Antibody
catalog :
100-4143
quantity :
2 mL
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
fission yeast, human
application :
western blot, ELISA, immunoprecipitation
more info or order :
Rockland Immunochemicals product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:10,000; loading ...; fig 5d
Cui Y, Parashar S, Zahoor M, Needham P, Mari M, Zhu M, et al. A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation. Science. 2019;365:53-60 pubmed publisher
product information
Catalog Number :
100-4143
Name :
Anti-ALCOHOL DEHYDROGENASE (Yeast) (RABBIT) Antibody - 100-4143
Display Name :
Alcohol Dehydrogenase Antibody
Application Note :
This antibody has been tested for use in ELISA and by western blot.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
90 mg/mL
Concentration Definition :
by Refractometry
Conjugation :
(None)
Size :
2 mL
Default Unit :
mL
ELISA Dilution :
1:5,000 - 1:25,000
Immunoprecipitation Dilution :
1:100
Western Blot Dilution :
1:500 - 1:2,000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
Antiserum
Gene Name :
ADH1
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
This antibody was prepared from whole rabbit serum produced by repeated immunizations with full length Alcohol Dehydrogenase isolated from yeast.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alcohol Dehydrogenase [Yeast].  Cross reactivity against Alcohol Dehydrogenase from most fungal sources is likely due to sequence homology as determined by BLAST analysis.   Cross reactivity with Alcohol Dehydrogenase from other sources is unknown.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
2.0 mL
Species Reactivity :
Saccharomyces cerevisiae (Baker's yeast)
Storage :
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
rabbit anti-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1
Target Species :
Yeast
Background :
Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone.  Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer.  This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization.  Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.
Immunogen Type :
Native Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
P00330
NCBI :
12643994
Primary Image Name :
Anti-Alcohol Dehydrogenase Antibody - Western Blot
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.