PDI Antibody | Rockland Immunochemicals 100-401-G29 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

PDI Antibody

catalog :

100-401-G29

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

100-401-G29

Name :

Anti-PDI (RABBIT) Antibody - 100-401-G29

Display Name :

PDI Antibody

Application Note :

Anti-PDI Antibody is suitable for use in WB, IP, IF microscopy and IHC. Expect a band approximately ~58kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Clonality :

Polyclonal

Concentration Value :

85 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

100 µL

Default Unit :

Immunohistochemistry Dilution :

User Optimized

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:4000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

P4hb

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

PDI Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against rat PDI synthetic peptide conjugated to KLH.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-PDI Antibody was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest cross-reactivity with PDI from Human, Mouse, Rat, Dog, Hamster, Monkey, Guinea pig, Bovine, Sheep, Pig, and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with PDI from other sources has not been determined. Chaperones, Organelle Markers research.

Species Reactivity :

Human, Mouse, Rat, Dog, Hamster, Monkey, Guinea pig, Bovine, Sheep, Pig, Xenopus

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

PDA2, PDI, PDIA2, PDIP, pancreatic protein disulfide isomerase, Protein disulfide-isomerase, PDI, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta

Target Species :

Rat

Background :

The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins. Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins. PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase. PDI is an abundant 55kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol. PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus. It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Immunohistochemistry,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P04785

NCBI :

NP_001099245.2

Primary Image Name :

PDI Western Blot

more info or order :

Rockland Immunochemicals product webpage