Hsc70 (Hsp73) Antibody | Rockland Immunochemicals 100-401-F49 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Hsc70 (Hsp73) Antibody

catalog :

100-401-F49

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

100-401-F49

Name :

Anti-Hsc70 (Hsp73) (RABBIT) Antibody - 100-401-F49

Display Name :

Hsc70 (Hsp73) Antibody

Application Note :

Anti-Hsc70 (Hsp73) Antibody is suitable for use in WB, IF, IP, IHC and blocking. Expect a band approximately ~73kDa protein corresponding to the molecular mass of hsc70 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Clonality :

Polyclonal

Concentration Value :

Neat

Conjugation :

(None)

Size :

100 µL

Default Unit :

Immunohistochemistry Dilution :

User Optimized

IF Microscopy Dilution :

1:250

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1: 1500-2000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

HSPA8

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Hsc70 (Hsp73) Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against a c-terminal region of human hsp73.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-Hsc70 (Hsp73) Antibody was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest cross-reactivity with Hsc70 (Hsp73) from Human and Hamster based on 100% homology with the immunizing sequence. Does not cross-react with hsp70. Cross-reactivity with Hsc70 (Hsp73) from other sources has not been determined. Heat Shock research.

Species Reactivity :

Human, Hamster

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Heat shock cognate protein 71-kDa antibody, Heat shock protein 8 antibody, Heat-shock70-KD protein 10, formerly antibody, HSC54 antibody, HSC71 antibody, Hsc54, Hsc71, Hsc73, Hsp71, Hsp73, HspA10, HspA8, LAP1, NIP71, Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8

Target Species :

Human

Background :

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. When cells are subjected to metabolic stress (e.g., heat shock) a member of the hsp 70 family, hsp 70 (hsp72), is expressed; hsp 70 is highly related to hsc70 (>90% sequence identity). Constitutively expressed hsc70 rapidly forms a stable complex with the highly inducible hsp70 in cells following heat shock. The interaction of hsc70 with hsp 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on hsc70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P11142

NCBI :

NP_006588.1

Primary Image Name :

Hsc70 (Hsp73) Immunofluorescence

more info or order :

Rockland Immunochemicals product webpage