Erk1/2 Antibody | Rockland Immunochemicals 100-401-F25 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Erk1/2 Antibody

catalog :

100-401-F25

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, flow cytometry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

100-401-F25

Name :

Anti-Erk1/2 (RABBIT) Antibody - 100-401-F25

Display Name :

Erk1/2 Antibody

Application Note :

Anti-Erk1/2 Antibody is suitable for use in WB, IF microscopy and IHC. Expect a band approximately ~44kda and ~42kDa bands corresponding to the molecular weights of Erk1 and Erk2. Specific conditions for reactivity should be optimized by the end user.

Clonality :

Polyclonal

Concentration Value :

85 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

100 µL

Default Unit :

Flow Cytometry Dilution :

10µg/mL

Immunohistochemistry Dilution :

1:50

Western Blot Dilution :

1:5000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

Mapk3

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Erk 1/2 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the synthetic peptide coupled to KLH.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-Erk1/2 Antibody was purified by affinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Erk1/2 from Human, Mouse, Rat, Cow, Sheep, Chicken, Drosophila, and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Erk1/2 from other sources has not been determined. Cell Signaling research.

Species Reactivity :

Human, Mouse, Rat, Bovine, Sheep, Chicken, Drosophila, Xenopus

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

Mitogen-activated protein kinase 3, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1, Erk1, Prkm3

Target Species :

Rat

Background :

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. They are both located in the cytosol and mitochondria. While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions, the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases. ERK1/2 activation is generally thought to confer a survival advantage to cells; however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Flow Cytometry,Immunohistochemistry,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P21708

NCBI :

NP_059043.1

Primary Image Name :

ERK 1/2 Immunofluorescence

more info or order :

Rockland Immunochemicals product webpage