Calreticulin Antibody | Rockland Immunochemicals 100-401-E99 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Calreticulin Antibody

catalog :

100-401-E99

quantity :

200 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, chicken, bovine

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

100-401-E99

Name :

Anti-Calreticulin (RABBIT) Antibody - 100-401-E99

Display Name :

Calreticulin Antibody

Application Note :

Anti-Calreticulin Antibody is suitable for use in WB, IHC, IF microscopy and IP. Expect a band approximately ~63kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Clonality :

Polyclonal

Concentration Value :

85 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

200 µL

Default Unit :

Immunohistochemistry Dilution :

User Optimized

IF Microscopy Dilution :

1:200

Immunoprecipitation Dilution :

User Optimized

Western Blot Dilution :

1:5000-10000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

CALR

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Calreticulin Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide of human calreticulin.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Purity and Specificity :

Anti-Calreticulin Antibody was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest cross-reactivity with Calreticulin from Human, mouse, rat, bovine, canine, chicken, guinea pig, monkey, pig, hamster, rabbit , and sheep based on 100% homology with the immunizing sequence. Cross-reactivity with Calreticulin from other sources has not been determined. Cell Signaling research.

Species Reactivity :

Human, mouse, rat, bovine, canine, chicken, guinea pig, monkey, swine, hamster, rabbit , sheep

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

CALR, Calregulin, cC1qR, CRP55, ERp60, HSCBP, RO, SSA, grp60, Calreticulin, Endoplasmic reticulum resident protein 60, CRTC

Target Species :

Human

Background :

Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus. CRT’s three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity. Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells, and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation. Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell. Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P27797

NCBI :

NP_004334.1

Primary Image Name :

Calreticulin Immunofluorescence

more info or order :

Rockland Immunochemicals product webpage