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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Neuron specific enolase (NSE) Antibody
catalog :
100-401-E00
quantity :
100 µL
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, chicken, bovine
application :
western blot
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
100-401-E00
Name :
Anti-Neuron specific enolase (NSE) (Rabbit) Antibody - 100-401-E00
Display Name :
Neuron specific enolase (NSE) Antibody
Application Note :
Anti-Neuron specific enolase (Rabbit) antibody is suitable for use in Western Blot and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47kDa in size corresponding to NSE protein by western blotting.
Clonality :
Polyclonal
Concentration Value :
Titrated value sufficient to run approximately 10 mini blots.
Conjugation :
(None)
Size :
100 µL
Default Unit :
ul
IF Microscopy Dilution :
1:500
Western Blot Dilution :
1:2000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
Antiserum
Gene Name :
ENO2
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-NSE Antibody was produced by repeated immunizations with recombinant human NSE expressed in E. coli.
Packing Type :
Dry Ice
Physical State :
Liquid
Purity and Specificity :
Anti-NSE antibody directed against human neuron specific enolase protein. This antibody is a monospecific neat serum. The antibody has been directly tested for reactivity in bovine, chicken, human, mouse, and rat. Cross reactivity with NSE from other species has not been determined but reactivity on other mammalian tissues is expected.
Species Reactivity :
bovine, chicken, human, mouse, rat
Storage :
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Synonyms :
Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, NSE
Target Species :
Human
Background :
NSE antibody detects NSE protein. Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. Anti-neuron specific enolase (NSE) antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Sample Size :
No
Application Text :
IF Microscopy,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
P09104
Primary Image Name :
Immunofluorescence Microscopy - Anti-NSE (Neuron specific enolase) antibody.
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.