product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
Histone H4 Antibody
catalog :
100-401-BR9
quantity :
100 µL
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
100-401-BR9
Name :
Anti-Histone H4 (RABBIT) Antibody - 100-401-BR9
Display Name :
Histone H4 Antibody
Application Note :
Anti-Histone H4 pan Antibody is suitable for Western Blot, Chromatin Immunoprecipitation, Dot Blots, and ELISA. Expect a single band approximately 11.3 kDa in size corresponding to H4 by western blot in the appropriate tissue or cell lysate. Specific conditions for reactivity should be optimized by the end user.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
90 mg/mL
Concentration Definition :
by Refractometry
Conjugation :
(None)
Size :
100 µL
Default Unit :
ul
ELISA Dilution :
1:20000
Western Blot Dilution :
1:500-1:2000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
Antiserum
Gene Name :
HIST1H4A
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
Anti-Histone H4 pan Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminal region of histone H4.
Other Performance Data :
Dot Blot: 1:1000
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Anti-Histone H4 pan Antibody antiserum is directed against Histone H4. Cross reactivity with other species was not tested.
Species Reactivity :
Human
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Subclass :
IgG
Synonyms :
rabbit anti-Histone H4 antibody, Histone H4, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4J, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4
Target Species :
Human
Background :
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.
Immunogen Type :
Peptide
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
N-Terminus
Application Text :
ELISA,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
P62805
NCBI :
NP_001029249
Primary Image Name :
Western Blot of Rabbit anti-Histone H4 antibody.
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.