Sars-Cov Nonstructural Protein 3 Antibody | Rockland Immunochemicals 100-401-A52 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Sars-Cov Nonstructural Protein 3 Antibody

catalog :

100-401-A52

quantity :

100 µL

price :

369.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

SARS coronavirus

application :

western blot, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

citations: 3

Reference
Oudshoorn D, Rijs K, Limpens R, Groen K, Koster A, Snijder E, et al. Expression and Cleavage of Middle East Respiratory Syndrome Coronavirus nsp3-4 Polyprotein Induce the Formation of Double-Membrane Vesicles That Mimic Those Associated with Coronaviral RNA Replication. MBio. 2017;8: pubmed publisher
de Wilde A, Raj V, Oudshoorn D, Bestebroer T, van Nieuwkoop S, Limpens R, et al. MERS-coronavirus replication induces severe in vitro cytopathology and is strongly inhibited by cyclosporin A or interferon-? treatment. J Gen Virol. 2013;94:1749-60 pubmed publisher
Snijder E, van der Meer Y, Zevenhoven Dobbe J, Onderwater J, van der Meulen J, Koerten H, et al. Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex. J Virol. 2006;80:5927-40 pubmed

product information

Catalog Number :

100-401-A52

Name :

Anti-SARS-CoV Nonstructural Protein 3 (nsp3) (RABBIT) Antibody - 100-401-A52

Display Name :

Sars-Cov Nonstructural Protein 3 Antibody

Application Note :

This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 211 kDa in size corresponding to SARS-CoV nsp3 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

Clonality :

Polyclonal

Concentration Value :

85 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

100 µL

Default Unit :

IF Microscopy Dilution :

1:1,000

Immunoprecipitation Dilution :

1:60

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This whole rabbit serum was produced by repeated immunizations with 3 E.coli derived proteins representing different parts of the SARS-CoV Nsp3 protein: aa 834-943 of polyprotein 1a (ADRP domain), aa 1208-1557 of polyprotein 1a (SARS-Unique Domain) and aa 1558-2040 of polyprotein 1a (Papain-like proteinase domain).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This antibody is directed against SARS-Coronavirus nsp3 protein. The product is neat antiserum. Cross reactivity with homologues from other sources has not been determined.

Species Reactivity :

SARS-Coronavirus nsp3 protein

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Sars-Cov Nonstructural Protein 3 Antibody, Replicase polyprotein 1a, pp1a antibody, nsp3, PL2-PRO, Papain-like proteinase, SARS coronavirus main proteinase

Target Species :

SARS

Background :

The nonstructural protein 3 (nsp3) is one of the SARS-Coronavirus replicase cleaving products, encoded by ORF1a. Nsp3 is thought to be part of the viral replication complex, which is associated with intracellular membranes. Nsp3 contains a papain-like cysteine proteinase and ADP-ribose-1'-monophosphatase activities. Anti-SARS-CoV Nonstructural Protein 3 (nsp3) Antibody is useful for researchers interested in viral research.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

IF Microscopy,Immunoprecipitation,Western Blot,

Other :

IMMUNOELECTRON MICROSCOPY 1:500

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

369.00 USD

UniProt :

P0C6U8

NCBI :

30124074

Primary Image Name :

Anti-SARS-CoV nsp3 Protein Antibody - Immunofluorescence Microscopy

more info or order :

Rockland Immunochemicals product webpage