Cul3 Antibody | Rockland Immunochemicals 100-401-A03 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Cul3 Antibody

catalog :

100-401-A03

quantity :

100 µL

price :

369.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

100-401-A03

Name :

Anti-Cul3 (N-terminal specific) (RABBIT) Antibody - 100-401-A03

Display Name :

Cul3 Antibody

Application Note :

This antibody reacts with human Cul3 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). An 88.9 kDa band corresponding to human Cul3 is detected. Most cell lines expressing Cul3 can be used as a positive control. Researchers should determine optimal titers for other applications.

Clonality :

Polyclonal

Concentration Value :

85 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:2,000 - 1:10,000

Immunohistochemistry Dilution :

User Optimized

Immunoprecipitation Dilution :

1:100

Western Blot Dilution :

1:500 - 1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

CUL3

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Cul3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal near amino acids 1-25 of Human Cul3 coupled to KLH.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-Cul3 antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 3. Cross reactivity is expected against mouse Cul3 based on sequence homology. Cross reactivity with other human cullins is unlikely based on sequence homology.

Species Reactivity :

human

Storage :

Store anti-Cul3 at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Cul3 Antibody, rabbit anti-Cullin3 antibody, KIAA0617 antibody, CUL, Cullin

Target Species :

Human

Background :

Cul3 antibody is ideal for Cell Biology, Cancer and Ubiquitin research. Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

369.00 USD

UniProt :

Q13618

NCBI :

4503165

Primary Image Name :

Anti-CUL3 Antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage