product summary
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company name :
Rockland Immunochemicals
product type :
antibody
product name :
PERK Antibody
catalog :
100-401-962
quantity :
100 µL
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
mouse
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation
more info or order :
Rockland Immunochemicals product webpage
citations: 5
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 4c
Mukhopadhyay C, Triplett A, Bargar T, HECKMAN C, Wagner K, Naramura M. Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation. Proc Natl Acad Sci U S A. 2016;113:E8228-E8237 pubmed publisher
  • western blot; mouse; 1:1000; fig 2e
Xu Z, Bu Y, Chitnis N, Koumenis C, Fuchs S, Diehl J. miR-216b regulation of c-Jun mediates GADD153/CHOP-dependent apoptosis. Nat Commun. 2016;7:11422 pubmed publisher
Srinivasan M, Bhopale K, Amer S, Wan J, Kaphalia L, Ansari G, et al. Linking Dysregulated AMPK Signaling and ER Stress in Ethanol-Induced Liver Injury in Hepatic Alcohol Dehydrogenase Deficient Deer Mice. Biomolecules. 2019;9: pubmed publisher
Maekawa T, Liu B, Nakai D, Yoshida K, Nakamura K, Yasukawa M, et al. ATF7 mediates TNF-α-induced telomere shortening. Nucleic Acids Res. 2018;46:4487-4504 pubmed publisher
Howley B, Link L, Grelet S, El Sabban M, Howe P. A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer. Oncogene. 2018;37:1308-1325 pubmed publisher
product information
Catalog Number :
100-401-962
Name :
Anti-PKR-like Endoplasmic Reticulum Kinase (PERK) (RABBIT) Antibody - 100-401-962
Display Name :
PERK Antibody
Application Note :
This antiserum has been tested for use in western blotting, immunoprecipitation and immunohistochemistry.  Specific conditions for reactivity should be optimized by the end user.  Expect bands approximately 150kDa by western blotting in the appropriate cell lysate or extract.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
80 mg/mL
Concentration Definition :
by Refractometry
Conjugation :
(None)
Size :
100 µL
Default Unit :
ul
ELISA Dilution :
1:4,000 - 1:20,000
Immunohistochemistry Dilution :
1:1,000
IF Microscopy Dilution :
User Optimized
Immunoprecipitation Dilution :
10-30ul
Western Blot Dilution :
1:500 – 1:3000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
Antiserum
Gene Name :
Eif2ak3
Host Animal :
Rabbit
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen :
This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse deltaN PERK.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This antiserum is directed against PERK and reacts with the PERK from mouse tissues. Reactivity to other species is unknown.
Species Reactivity :
mouse
Storage :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
rabbit anti-PKR-like Endoplasmic Reticulum Kinase Antibody, rabbit anti-PERK Antibody, PKR-like endoplasmic reticulum kinase, PERK, Eukaryotic translation initiation factor 2-alpha kinase 3
Target Species :
Mouse
Background :
The PKR-like endoplasmic reticulum kinase (PERK, also know as Eukaryotic translation initiation factor 2-alpha kinase 3) is a type I transmembrane protein localized to the endoplasmic reticulum (ER). PERK consists of an N-terminal ER luminal domain, a membrane-spanning region, and a cytosolic C-terminal serine/threonine kinase domain (1). The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells (2). PERK activation occurs upon accumulation of misfolded proteins and the ER lumen, which triggers GRP78 dissociation from PERK thereby allowing PERK dimerization and autophosphorylation (3, 4). PERK phosphorylates two established targets: the eukaryotic translation initiation factor 2 alpha (eIF2, (1)) and the Nrf2 transcription factor (5). Phosphorylation of eIF2 results in attenuation of translation initiation (6). The translational block also contributes to cell cycle arrest due to loss of the G1 regulatory protein, cyclin D1 (7). PERK-dependent phosphorylation of Nrf2 promotes transcription of phase II detoxifying enzymes which is critically important for elimination of intracellular reactive oxygen species (8). Thus, while inhibiting new protein synthesis and thereby decreasing the ER protein load PERK simultaneously induces expression of genes that help restore cellular redox homeostasis and promote survival.
Immunogen Type :
Recombinant Protein
Low Endotoxin :
No
Sample Size :
No
Target Domain of Reactivity :
Internal
Application Text :
ELISA,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Primary Antibodies
Conjugation Name :
Unconjugated
UniProt :
Q9Z2B5
NCBI :
32451785
Primary Image Name :
Anti-PERK Antibody - Western Blot
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.