NOTCH 1 Antibody | Rockland Immunochemicals 100-401-405 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

NOTCH 1 Antibody

catalog :

100-401-405

quantity :

200 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

100-401-405

Name :

Anti-NOTCH 1 (intra) (RABBIT) Antibody - 100-401-405

Display Name :

NOTCH 1 Antibody

Application Note :

Anti-Notch-1 has been tested in IHC and ELISA. This antibody is useful in western blot. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

75 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

200 µL

Default Unit :

ELISA Dilution :

1:20,000 - 1:100,000

Immunohistochemistry Dilution :

1:1,000 - 1:5,000

Western Blot Dilution :

1:2,000 - 1:10,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

NOTCH1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-Notch antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid 2480-2510 of human Notch 1. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Notch1 antibody is directed against human NOTCH 1. No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-notch1 antibody, hN1 antibody, Neurogenic locus Notch homolog protein 1 antibody, Notch 1 intracellular domain antibody, Notch homolog 1 translocation associated antibody, TAN1 antibody, Translocation associated Notch protein TAN1 antibody

Target Species :

Human

Background :

Notch-1 is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Anti-Notch 1 Antibody is useful for researchers interested in Notch pathways, cancer research, transcription factors, and DNA binding research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P46531

NCBI :

CAG33502.1

Primary Image Name :

Anti-NOTCH 1 Antibody - Immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage