NFkB p65 (RelA) Phospho S276 Antibody | Rockland Immunochemicals 100-401-264 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

NFkB p65 (RelA) Phospho S276 Antibody

catalog :

100-401-264

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 6

Reference
Lai L, Azzam K, Lin W, Rai P, Lowe J, Gabor K, et al. MicroRNA-33 Regulates the Innate Immune Response via ATP Binding Cassette Transporter-mediated Remodeling of Membrane Microdomains. J Biol Chem. 2016;291:19651-60 pubmed publisher
Zhu J, Carver W. Effects of interleukin-33 on cardiac fibroblast gene expression and activity. Cytokine. 2012;58:368-79 pubmed publisher
Spooren A, Kolmus K, Vermeulen L, Van Wesemael K, Haegeman G, Gerlo S. Hunting for serine 276-phosphorylated p65. J Biomed Biotechnol. 2010;2010:275892 pubmed publisher
Begley L, Kasina S, Mehra R, Adsule S, Admon A, Lonigro R, et al. CXCL5 promotes prostate cancer progression. Neoplasia. 2008;10:244-54 pubmed
Begley L, Monteleon C, Shah R, MacDonald J, Macoska J. CXCL12 overexpression and secretion by aging fibroblasts enhance human prostate epithelial proliferation in vitro. Aging Cell. 2005;4:291-8 pubmed
Chapman N, Europe Finner G, Robson S. Expression and deoxyribonucleic acid-binding activity of the nuclear factor kappaB family in the human myometrium during pregnancy and labor. J Clin Endocrinol Metab. 2004;89:5683-93 pubmed

product information

Catalog Number :

100-401-264

Name :

Anti-NFKB p65 (Rel A) pS276 (RABBIT) Antibody - 100-401-264

Display Name :

NFkB p65 (RelA) Phospho S276 Antibody

Application Note :

Phospho NFkB antibody reacts human pS276 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. A 1:500 dilution has been used for staining p65 in human kidney tissue by IHC. Tissue was formalin fixed and paraffin embedded. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. All conditions must be user optimized.

Clonality :

Polyclonal

Concentration Value :

80 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:10,000 - 1:30,000

Immunohistochemistry Dilution :

1:200-1:800

Western Blot Dilution :

1:1,000 - 1:5,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

RELA

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

NFkB p65 (Rel A) peptide corresponding to an internal region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-NFkB p65 (RelA) Phospho S276 Antibody was prepared from monospecific antiserum by delipidation and defibrination. This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated p65 is minimal. Cross reactivity with pS276 phosphorylated p65 from mouse, rat or other species has not been determined.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-NFkB p65 pS276 Antibody, rabbit anti-RelA pS276 Antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Anti-NF-kB antibody

Target Species :

Human

Background :

NF-kB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NF-kB bound to IkB. NF-kB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), cRel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NF-kB subunit p65, similar to p50/p65 heterodimers. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-alpha. IkB-alpha binds to the p65 subunit preventing nuclear localization and DNA binding. Activators mediate a rapid phosphorylation of IkB by IkB kinase (IKK) which results in subsequent ubiquitination and proteolytic degradation. NF-kB is then transported to the nucleus, where it activates transcription of target genes through binding to NF-kB target sequences within the promoter. The HTLV-I protein Tax can induce constitutive NF-kB activation through phosphorylation of both IkB-alpha and IkB-beta. The transforming protein Tax inhibits p53 transcriptional activity through the NFkB signaling pathway, specifically via the p65 (RelA) subunit. The inhibition of p53 activity is dependent upon phosphorylation of p65 (RelA) at S536 by the upstream kinase IKKβ. Anti-NFkB antibody is ideal for Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q04206

NCBI :

223468676

Primary Image Name :

Immunohistochemistry - Anti-NFKB p65 (Rel A) pS276 Antibody

more info or order :

Rockland Immunochemicals product webpage