CIITA Antibody | Rockland Immunochemicals 100-401-249 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CIITA Antibody

catalog :

100-401-249

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, chromatin immunoprecipitation, ChIP-Seq

more info or order :

Rockland Immunochemicals product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; human; fig 4a chromatin immunoprecipitation; human; fig 4c	Su C, Lu F, Soldan S, Lamontagne R, Tang H, Napoletani G, et al. EBNA2 driven enhancer switching at the CIITA-DEXI locus suppresses HLA class II gene expression during EBV infection of B-lymphocytes. PLoS Pathog. 2021;17:e1009834 pubmed publisher
	Majumder P, Lee J, Rahmberg A, Kumar G, Mi T, Scharer C, et al. A super enhancer controls expression and chromatin architecture within the MHC class II locus. J Exp Med. 2020;217: pubmed publisher
	Scharer C, Choi N, Barwick B, Majumder P, Lohsen S, Boss J. Genome-wide CIITA-binding profile identifies sequence preferences that dictate function versus recruitment. Nucleic Acids Res. 2015;43:3128-42 pubmed publisher

product information

Catalog Number :

100-401-249

Name :

Anti-CIITA aa 1-333 (RABBIT) Antibody - 100-401-249

Display Name :

CIITA Antibody

Application Note :

For immunoblotting a 1:500 dilution is recommended. Researchers should determine optimal titers for other applications.

Clonality :

Polyclonal

Concentration Value :

90 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:5,000 - 1:25,000

Western Blot Dilution :

1:500 - 1:3,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

CIITA

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

The immunogen used for this study was a bacterially produced recombinant FLAG-CIITA corresponding to amino acids 1 through 333 of the human protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-CIITA antibody was prepared from monospecific antiserum by delipidation and defibrination.

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-CIITA Antibody, MHC class II transactivator, CIITA, MHC2TA

Target Species :

Human

Background :

Anti-CIITA antibody detects CIITA. The transactivator CIITA regulates basal and interferon-induced expression of Major Histocompatibility Complex class II genes. CIITA restores expression of all MHC class II gene expression in mutant cells and corrects regulatory defects of MHC class II genes. Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression. Antigen can be obtained from Raji cell lysates. Typically levels of CIITA expression are too low to detect endogenous levels of protein expression. Transiently transfected cells are usually employed to study this transcription factor.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P33076

NCBI :

P33076.2

Primary Image Name :

Anti-CIITA (1-333) Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage