Smac/Diablo Antibody | Rockland Immunochemicals 100-401-244 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Smac/Diablo Antibody

catalog :

100-401-244

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

100-401-244

Name :

Anti-Smac/DIABLO (RABBIT) Antibody - 100-401-244

Display Name :

Smac/Diablo Antibody

Application Note :

This antibody reacts with human Smac/DIABLO protein by immunoblot. Lysates from human HeLa and LNCaP cells are positive for Smac/DIABLO. Other animal tissues have not been tested.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

85 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:5,000 - 1:20,000

Immunoprecipitation Dilution :

1:100

Western Blot Dilution :

1:1,000 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

DIABLO

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This whole rabbit serum was prepared by repeated immunizations with recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This antiserum is directed against human Smac/DIABLO and is useful in determining its presence in various assays.

Species Reactivity :

Human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-smac antibody, rabbit anti-Diablo antibody, Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, Smac protein

Target Species :

Human

Background :

Apoptosis is a conserved cell suicide program essential for the development and homeostasis of multi-cellular organisms. Abnormal inhibition of apoptosis is a hallmark of cancer and autoimmune diseases, whereas excessive cell death is found in neurodegenerative disorders such as Alzheimers disease. Executioners of the apoptotic program are cysteine proteases termed caspases that exist as inactive zymogens in living cells and are activated during apoptosis. Active caspases cleave key intracellular protein substrates, resulting in the characteristic morphological changes associated with apoptosis. The release of cytochrome c from the mitochondria triggers the oligomerization of Apaf-1 in an ATP/dATP-dependent manner and induces the autoactivation of caspase-9. Active caspase-9 in turn activates downstream effector caspases including caspase -3, -6 and -7.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

Q9NR28

NCBI :

Q9NR28.1

Primary Image Name :

Anti-Smac Antibody - Western Blot

more info or order :

Rockland Immunochemicals product webpage