Dab1 Antibody | Rockland Immunochemicals 100-401-225 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Dab1 Antibody

catalog :

100-401-225

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:5000; loading ...; fig 3a	Fairchild C, Hino K, Han J, Miltner A, Peinado Allina G, Brown C, et al. RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion. Development. 2018;145: pubmed publisher
	Reyes R, Hino K, Canales C, Dickson E, La Torre A, Simo S. The E3 Ubiquitin Ligase CRL5 Regulates Dentate Gyrus Morphogenesis, Adult Neurogenesis, and Animal Behavior. Front Neurosci. 2022;16:908719 pubmed publisher
	Meseke M, Neumüller F, Brunne B, Li X, Anstötz M, Pohlkamp T, et al. Distal Dendritic Enrichment of HCN1 Channels in Hippocampal CA1 Is Promoted by Estrogen, but Does Not Require Reelin. Eneuro. 2018;5: pubmed publisher

product information

Catalog Number :

100-401-225

Name :

Anti-Dab1 (400-555) (RABBIT) Antibody - 100-401-225

Display Name :

Dab1 Antibody

Application Note :

Anti-Dab1 antibody is suitable for the detection of Dab1 by immunoprecipitation, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at 80 kDa corresponding to Dab1 in the appropriate tissue extract or cell lysate. For western blotting block the blot using 5% BLOTTO for 1 h at room temperature and followed by incubation with the primary antibody diluted in 1% BLOTTO in TTBS for 1 h at room temperature. For immunoprecipitation use 1 µl of antiserum per 500 µg of brain lysate. Perform immunoprecipitation at 4°C for 2 h. For immunoprecipitation buffer lysates with 50 mM Tris-Cl, pH 7.4, supplemented with 150 mM sodium chloride, 1% (v/v) NP-40, 10 µg/ml aprotinin and 10 µg/ml leupeptin.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

75 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:10,000 - 1:50,000

Immunohistochemistry Dilution :

1:5,000

Immunoprecipitation Dilution :

1 µl per 500 µg

Western Blot Dilution :

1:5,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

Dab1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of murine Dab1 at amino acids 400-555.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Dab-1 whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. This antibody is directed against Dab1 from mouse. Cross-reactivity with other species has not been determined. No reaction occurs with human or mouse Dab2.

Species Reactivity :

mouse

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Dab 1 antibody, Disabled homolog 1 antibody, Disabled homolog 1 Drosophila antibody, Scm antibody, Scr antibody, Scrambler antibody, Yot antibody, Yotari antibody

Target Species :

Mouse

Background :

Anti-Dab1 Antibody recognizes Dab1 that is a phosphoprotein encoded by the mouse gene dab and is related to the Drosophila gene 'disabled'. Mutations in the mouse dab gene may result in the 'scrambler' and 'yotari' phenotypes. Dab1 binds to non-receptor tyrosine kinases and plays an important role in brain development. Dab1 is expressed in neuronal populations exposed to reelin, and it functions as a signaling molecule that regulates cell positioning in the developing brain. Cloning of human Dab1 and sequence determinations suggest a 96% identity to the mouse sequence. Dab1 binds to cytoplasmic regions of very low density lipoprotein receptors (VLDLR), apolipoprotein E receptor-2 (ApoER2) and the Amyloid Precursor Protein (APP) family of proteins. Dab1 accumulates in ectopic neurons from mice lacking Reelin or both VLDLR and ApoER2. In humans, Dab1 has been mapped to lp32-p312. This region shows homology of synteny with the segment of mouse chromosome 4 containing Dab1.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P97318

NCBI :

NP_034144.1

Primary Image Name :

IHC - Dab1 Antibody

more info or order :

Rockland Immunochemicals product webpage