antibody

Gli1 Antibody

100-401-223S

25 µL

99.00 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA

Anti-Gli1 (RABBIT) Antibody - 100-401-223S

Gli1 Antibody

This antibody has been tested for use in ELISA, Immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Gli-1 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, perform heat mediated antigen retrieval via the microwave method before commencing with staining.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Polyclonal

75 mg/ml

by Refractometry

(None)

25 µL

ul

1:20,000 - 1:100,000

1:500 - 1:2,000

1:2,000 - 1:10,000

Expiration date is one (1) year from date of opening.

Antiserum

GLI1

Rabbit

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

The whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acids 805-820 of mouse Gli-1. The peptide was synthesized as a multiple antigen peptide (MAP).

Dry Ice

Liquid (sterile filtered)

0.01% (w/v) Sodium Azide

This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is observed against Mouse and Human Gli-1. Cross-reactivity with other species is likely but has not been determined. No reaction occurs with human or mouse Gli-2 or Gli-3.

mouse, human

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

rabbit anti-Gli-1 Antibody, rabbit anti-Gli1 Antibody, Zinc finger protein GLI1 antibody, Glioma-associated oncogene antibody, Oncogene GLI antibody

Mouse

Anti Gli1 Antibody was produced against a peptide corresponding to the carboxy-terminal region of the mouse Gli-1 protein. This region of Gli1 is not conserved among other gli family members, namely Gli-2 and Gli-3. Gli was termed by Kinzler et al. (1987) as 'glioma-associated oncogene' amplified in malignant gliomas. Analysis of the cloned gene demonstrates that the gene contains 5 repeats of zinc-finger sequences, which places Gli in the family of Kruppel (Kr) zinc finger proteins. Northern analysis reveals that Gli is expressed in embryonal carcinoma cells but not in most adult tissue. Gli has been localized to 12q13-q14.3 by Southern blot analysis. In mice, the gene is located on chromosome 10. In mice, three zinc finger transcription factors, Gli-1, Gli-2 and Gli-3, have been implicated in the transduction of Sonic hedgehog (Shh) signal. In papillary epithelium, shh, gli1 and ptc all follow similar expression patterns. Gli-1 expression is central and probably sufficient for tumor development in humans.

Hall, J.M., Hooper, J.E., Finger, T.E. (1999) Expression of sonic hedgehog, patched, and gli1 in developing taste papillae of the mouse. J. Comp. Neurol. 406(2):143-155. Park, H. L. et. al. (2000) Mouse Gli1 mutants are viable but have defects in SHH signaling in combination with a Gli2 mutation. Development 127(8): 1593-1605. Kinzler, K.W. et. al. (1987) Identification of an amplified, highly expressed gene in a human glioma. Science 236:70-73.

Wetmore, C., Eberhardt, D. and Curran, T. (2000) The normal patched allele is expressed in medulloblastomas from mice with heterozygous germline mutation of patched. Cancer Res. 60(8): 2239-2246. Qualtrough D et al. (2004) Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment. Int J Cancer 110:831-7. Niemann C et al. (2003) Indian hedgehog and beta-catenin signaling: role in the sebaceous lineage of normal and neoplastic mammalian epidermis. Proc Natl Acad Sci U S A 100 Suppl 1:11873-80.

Peptide

No

Yes

Internal

ELISA,IF Microscopy,Western Blot,

User Optimized

Primary Antibodies

Unconjugated

99.00 USD

P47806

4885279

Anti-Gli-1 Antibody - Immunofluorescence