Gli1 Antibody | Rockland Immunochemicals 100-401-223 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Gli1 Antibody

catalog :

100-401-223

quantity :

100 µL

price :

369.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Reference
Armas López L, Pina Sanchez P, Arrieta O, de Alba E, Ortiz Quintero B, Santillan Doherty P, et al. Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients. Oncotarget. 2017;8:67056-67081 pubmed publisher

product information

Catalog Number :

100-401-223

Name :

Anti-Gli1 (RABBIT) Antibody - 100-401-223

Display Name :

Gli1 Antibody

Application Note :

This antibody has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Gli-1 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, perform heat mediated antigen retrieval via the microwave method before commencing with staining.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

75 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:20,000 - 1:100,000

Immunohistochemistry Dilution :

1:500 - 1:2,000

IF Microscopy Dilution :

1:500 - 1:2,000

Western Blot Dilution :

1:2,000 - 1:10,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

Gli1

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

The whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acids 805-820 of mouse Gli-1. The peptide was synthesized as a multiple antigen peptide (MAP).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is observed against Mouse and Human Gli-1. Cross-reactivity with other species is likely but has not been determined. No reaction occurs with human or mouse Gli-2 or Gli-3.

Species Reactivity :

mouse, human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-Gli-1 Antibody, rabbit anti-Gli1 Antibody, Zinc finger protein GLI1 antibody, Glioma-associated oncogene antibody, Oncogene GLI antibody

Target Species :

Mouse

Background :

Anti Gli1 Antibody was produced against a peptide corresponding to the carboxy-terminal region of the mouse Gli-1 protein. This region of Gli1 is not conserved among other gli family members, namely Gli-2 and Gli-3. Gli was termed by Kinzler et al. (1987) as 'glioma-associated oncogene' amplified in malignant gliomas. Analysis of the cloned gene demonstrates that the gene contains 5 repeats of zinc-finger sequences, which places Gli in the family of Kruppel (Kr) zinc finger proteins. Northern analysis reveals that Gli is expressed in embryonal carcinoma cells but not in most adult tissue. Gli has been localized to 12q13-q14.3 by Southern blot analysis. In mice, the gene is located on chromosome 10. In mice, three zinc finger transcription factors, Gli-1, Gli-2 and Gli-3, have been implicated in the transduction of Sonic hedgehog (Shh) signal. In papillary epithelium, shh, gli1 and ptc all follow similar expression patterns. Gli-1 expression is central and probably sufficient for tumor development in humans.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2020 Price :

369.00 USD

UniProt :

P47806

NCBI :

4885279

Primary Image Name :

Anti-Gli-1 Antibody - Immunofluorescence

more info or order :

Rockland Immunochemicals product webpage