RFX5 Antibody | Rockland Immunochemicals 100-401-194 product information

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

RFX5 Antibody

catalog :

100-401-194

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunoprecipitation

more info or order :

Rockland Immunochemicals product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; fig 8	Beaulieu Y, Leon Machado J, Ethier S, Gaudreau L, Steimle V. Degradation, Promoter Recruitment and Transactivation Mediated by the Extreme N-Terminus of MHC Class II Transactivator CIITA Isoform III. PLoS ONE. 2016;11:e0148753 pubmed publisher

product information

Catalog Number :

100-401-194

Name :

Anti-RFX5 (RABBIT) Antibody - 100-401-194

Display Name :

RFX5 Antibody

Application Note :

Anti-RFX5 Antibody was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 µl per assay. Specific conditions should be optimized by user. Anti-RFX5 Antibody was assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG. Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5. Other assays should be optimized by researcher.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

85 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

100 µL

Default Unit :

ELISA Dilution :

1:5,000 - 1:25,000

Western Blot Dilution :

1:500 - 1:3,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

RFX5

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

RFX5 peptide corresponding to amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-RFX5 Antibody was prepared by repeated immunizations of an RFX5 peptide conjugate and purified as monospecific antiserum after delipidation and defibrination.

Species of Origin :

human

Species Reactivity :

human

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Synonyms :

rabbit anti-RFX5 Antibody, DNA binding protein RFX5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, MHC

Target Species :

Human

Background :

RFX5 Antibody detects the RFX5 protein. Regulatory factor X subunit 5 (RFX5) is a member of a family of DNA-binding proteins that share a novel and highly characteristic DNA-binding domain called the RFX motif. It mediates cooperative binding between RFX and NF-Y, recognizes X-boxes, and activates transcription from class II MHC promoters. RFX5 mutations are seen in cases of a severe immunodeficiency syndrome called MHC-II deficiency (also known as bare lymphocyte syndrome (BLS)). These mutations prevent the RFX complex from binding to the X box in MHC-II promoters, resulting in a lack of MHC-II expression. Anti-RFX5 antibody is ideal for investigators involved in cytokines and growth factor research.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

UniProt :

P48382

NCBI :

CAO72162.1

more info or order :

Rockland Immunochemicals product webpage