cdk2 Antibody | Rockland Immunochemicals 100-401-161S product information

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product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

cdk2 Antibody

catalog :

100-401-161S

quantity :

25 µL

price :

99.00 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

product information

Catalog Number :

100-401-161S

Name :

Anti-cdk2 (RABBIT) Antibody - 100-401-161S

Display Name :

cdk2 Antibody

Application Note :

Anti-cdk2 antibody is suitable for immunohistochemistry, western blotting and other antibody based assays. Multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Use paraffin embedded tissues for immunohistochemistry. Optimal titers for other applications should be determined by the researcher.

Clonality :

Polyclonal

Concentration Value :

80 mg/mL

Concentration Definition :

by Refractometry

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:1,000 - 1:5,000

Immunohistochemistry Dilution :

1:100

IF Microscopy Dilution :

1:200 - 1:1,000

Immunoprecipitation Dilution :

1:100

Western Blot Dilution :

1:200 - 1:1,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

Antiserum

Gene Name :

CDK2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-cdk2 antibody was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 33 kDa cdk2 protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk2 from other species may also occur.

Species Reactivity :

human, rat, mouse

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

rabbit anti-Cdk2 Antibody, Cdc2 related protein kinase antibody, Cdk 2 antibody, CDK2 antibody, Cell division kinase 2 antibody, Cell division protein kinase 2 antibody, rabbit anti-Cyclin dependent kinase 2 antibody, p33 protein kinase antibody, cdk

Target Species :

Human

Background :

Anti cdk2 antibody recognizes a protein encoded by a gene member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

General Reference :

Elledge, S.J. and Spotswood, M. (1991) A new human p34 protein kinase CDK2, identified by complementation of a cdc28 mutation, is a homolog of Xenopus Eg1. EMBO J. 10; 2653-2659. Giordano, A., Lee, J.H., Scheppler, J.A., Herrmann, C., Harlow, E., Deuschle, U., Beach, D. and Franza, B. Jr. (1991) Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A. Science 253; 1271-1275. Tsai, L.H., Harlow, E. and Myerson, M. (1991) Isolation of the human CDK2 gene that encodes the cyclin A and adenovirus E1A-associated p33 kinase. Nature 353; 174-177.

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

C-Terminus

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot,

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

Q9BW66

NCBI :

CAA43985.1

Primary Image Name :

Anti-cdk2 Antibody - Western Blot