Llama IgG3 isotype control Fluorescein | Rockland Immunochemicals 025-0243 product information

product summary

company name :

Rockland Immunochemicals

product type :

protein

product name :

Llama IgG3 isotype control Fluorescein

catalog :

025-0243

quantity :

100 µg

more info or order :

Rockland Immunochemicals product webpage

product information

Catalog Number :

025-0243

Name :

Llama IgG3 isotype control Fluorescein Conjugated - 025-0243

Display Name :

Llama IgG3 isotype control Fluorescein

Application Note :

Llama IgG3 FITC conjugated isotype control can be utilized as a control or standard reagent in Flow Cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting experiments where determination of sample isotype is important.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Concentration Value :

1mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

FITC

Size :

100 µg

Default Unit :

µg

FLISA Dilution :

User Optimized

Flow Cytometry Dilution :

User Optimized

IF Microscopy Dilution :

User Optimized

Western Blot Dilution :

User Optimized

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG3

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Packing Type :

Ambient

Physical State :

Lyophilized

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Llama IgG3 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced unconjugated llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa. No bands corresponding to unconjugated llama IgG1 or IgG2 are observed. Fluorescein Conjugated Llama IgG3 was proven by Dot Blot.

Reconstitution Buffer :

Restore with deionized water (or equivalent)

Reconstitution Volume :

100 µL

Species of Origin :

Llama

Stabilizer :

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage :

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Subclass :

IgG3

Synonyms :

Llama IgG3 isotype, Llama IgG31 subclass isotype, Llama IgG31 Fluorescein Conjugated, Llama IgG3 FITC, Llama isotype control

Background :

Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35% - 70% of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa. In llama and other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses of gamma chain antibodies.

Low Endotoxin :

PSC Format :

Native Protein

PSC Type :

Protein

Sample Size :

Application Text :

FLISA,Flow Cytometry,IF Microscopy,Western Blot,

Category :

Proteins and Peptides

Conjugation Name :

Fluorescein (FITC)

more info or order :

Rockland Immunochemicals product webpage