product summary
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company name :
Rockland Immunochemicals
product type :
protein
product name :
Mouse IgG2a isotype control Phycoerythrin
catalog :
010-0841
quantity :
1 mL
more info or order :
product information
Catalog Number :
010-0841
Name :
MOUSE IgG2a isotype control Phycoerythrin conjugated - 010-0841
Display Name :
Mouse IgG2a isotype control Phycoerythrin
Application Note :
Mouse IgG2a isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
M2A
Concentration Value :
1.0 mg/mL
Concentration Definition :
by absorbance = 82.0 at 565 nm
Conjugation :
RPE
Size :
1 mL
Default Unit :
mL
ELISA Dilution :
Yes
FLISA Dilution :
User Optimized
Flow Cytometry Dilution :
1:1000-1:5000
IF Microscopy Dilution :
1:1000-1:5000
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG2a
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
This product was prepared from ascitic fluid by affinity chromatography. Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Phycoerythrin and Anti-Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other anti-Mouse heavy or light chain isotypes antibodies.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
1.0 mL
Species of Origin :
Mouse
Species Reactivity :
mouse
Stabilizer :
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Storage :
Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Subclass :
IgG2a
Synonyms :
Phycoerythrin conjugated Mouse IgG2a isotype control, Mouse IgG2a subclass isotype control PE conjugation
Background :
Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Low Endotoxin :
No
PSC Format :
Native Protein
PSC Type :
Protein
Sample Size :
No
Application Text :
ELISA,FLISA,Flow Cytometry,IF Microscopy,
Other :
User Optimized
Category :
Proteins and Peptides
Conjugation Name :
R-Phycoerythrin (RPE)
Primary Image Name :
Dot Blot results of Mouse IgG2a R-Phycoerythrin conjugate
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.