product summary
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company name :
Rockland Immunochemicals
product type :
protein
product name :
Mouse IgG3 isotype Control
catalog :
010-0143
quantity :
1 mg
more info or order :
product information
Catalog Number :
010-0143
Name :
MOUSE IgG3 isotype control - 010-0143
Display Name :
Mouse IgG3 isotype Control
Application Note :
Mouse IgG3 isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Monoclonal
Clone ID :
MG3
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
1 mg
Default Unit :
mg
ELISA Dilution :
User Optimized
FLISA Dilution :
User Optimized
Flow Cytometry Dilution :
1:1000-1:5000
IF Microscopy Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgG3
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Extensive cross-adsorbtion was performed to remove any unwanted subclasses. Typically less than 1% cross reactivity against other mouse and human heavy or light chains isotypes was detected by ELISA.
Species of Origin :
Mouse
Storage :
Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Subclass :
IgG3
Synonyms :
MOUSE IgG3 subclass isotype control, MOUSE IgG3 isotype control
Background :
Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Low Endotoxin :
No
PSC Format :
Native Protein
PSC Type :
Protein
Sample Size :
No
Application Text :
ELISA,FLISA,Flow Cytometry,IF Microscopy,Western Blot,
Other :
User Optimized
Category :
Proteins and Peptides
Conjugation Name :
Unconjugated
Primary Image Name :
Mouse IgG3 SDS
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.