product summary
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company name :
Rockland Immunochemicals
product type :
protein
product name :
Mouse IgG
catalog :
010-0102-0005
quantity :
5
price :
239.00 USD
citations: 14
Reference
Wedegaertner H, Bosompra O, Kufareva I, Trejo J. Divergent regulation of α-arrestin ARRDC3 function by ubiquitination. Mol Biol Cell. 2023;34:ar93 pubmed publisher
Neumeier D, Yermanos A, Agrafiotis A, Csepregi L, Chowdhury T, Ehling R, et al. Phenotypic determinism and stochasticity in antibody repertoires of clonally expanded plasma cells. Proc Natl Acad Sci U S A. 2022;119:e2113766119 pubmed publisher
Virtakoivu R, Rannikko J, Viitala M, Vaura F, Takeda A, L xf6 nnberg T, et al. Systemic Blockade of Clever-1 Elicits Lymphocyte Activation Alongside Checkpoint Molecule Downregulation in Patients with Solid Tumors: Results from a Phase I/II Clinical Trial. Clin Cancer Res. 2021;27:4205-4220 pubmed publisher
Mason D, Friedensohn S, Weber C, Jordi C, Wagner B, Meng S, et al. Optimization of therapeutic antibodies by predicting antigen specificity from antibody sequence via deep learning. Nat Biomed Eng. 2021;5:600-612 pubmed publisher
Arakaki A, Pan W, Wedegaertner H, Roca Mercado I, Chinn L, Gujral T, et al. α-Arrestin ARRDC3 tumor suppressor function is linked to GPCR-induced TAZ activation and breast cancer metastasis. J Cell Sci. 2021;134: pubmed publisher
Fair Mäkelä R, Ugur M, Iftakhar E Khuda I, Kähäri L, Kukkonen Macchi A, Brenyah H, et al. Robo4 contributes to the turnover of Peyer's patch B cells. Mucosal Immunol. 2020;13:245-256 pubmed publisher
Zhang Z, So K, Peterson R, Bauer M, Ng H, Zhang Y, et al. Elav-Mediated Exon Skipping and Alternative Polyadenylation of the Dscam1 Gene Are Required for Axon Outgrowth. Cell Rep. 2019;27:3808-3817.e7 pubmed publisher
Matsumura T, Ikebe T, Arikawa K, Hosokawa M, Aiko M, Iguchi A, et al. Sequential Sensing by TLR2 and Mincle Directs Immature Myeloid Cells to Protect against Invasive Group A Streptococcal Infection in Mice. Cell Rep. 2019;27:561-571.e6 pubmed publisher
Kawakami Y, Kurihara Y, Saito Y, Fujita Y, Yamashita T, Takei K. The Soluble Form of LOTUS inhibits Nogo Receptor-Mediated Signaling by Interfering with the Interaction Between Nogo Receptor Type 1 and p75 Neurotrophin Receptor. J Neurosci. 2018;38:2589-2604 pubmed publisher
Kruse N, Schlossmacher M, Schulz Schaeffer W, Vanmechelen E, Vanderstichele H, El Agnaf O, et al. A First Tetraplex Assay for the Simultaneous Quantification of Total α-Synuclein, Tau, β-Amyloid42 and DJ-1 in Human Cerebrospinal Fluid. PLoS ONE. 2016;11:e0153564 pubmed publisher
Bronietzki M, Kasmapour B, Gutierrez M. Study of phagolysosome biogenesis in live macrophages. J Vis Exp. 2014;: pubmed publisher
Li D, Liu S, Liu R, Park R, Hughes L, Krasnoperov V, et al. Targeting the EphB4 receptor for cancer diagnosis and therapy monitoring. Mol Pharm. 2013;10:329-36 pubmed publisher
Penaranda C, Tang Q, Bluestone J. Anti-CD3 therapy promotes tolerance by selectively depleting pathogenic cells while preserving regulatory T cells. J Immunol. 2011;187:2015-22 pubmed publisher
Ono K, Yu R, Mohri K, Ono S. Caenorhabditis elegans kettin, a large immunoglobulin-like repeat protein, binds to filamentous actin and provides mechanical stability to the contractile apparatuses in body wall muscle. Mol Biol Cell. 2006;17:2722-34 pubmed
image
image 1 :
Rockland Immunochemicals 010-0102-0005 image 1
SDS-Page of Mouse IgG whole molecule. Lane 1: Mouse IgG reduced. Lane 2: Molecular Weight Marker. Lane 3: Mouse IgG non-reduced. Load: 1 µg per lane. Predicted/Observed size (non-reduced): 160 kDa, 160 kDa. Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa.
product information
Catalog Number :
010-0102-0005
Name :
Mouse IgG Whole Molecule - 010-0102-0005
Display Name :
Mouse IgG
Application Note :
Mouse IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration Value :
10.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
5
Default Unit :
mg
ELISA Dilution :
User Optimized
Flow Cytometry Dilution :
User Optimized
Immunohistochemistry Dilution :
User Optimized
Immunoprecipitation Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is one (1) year from date of receipt.
Format :
IgG
Harmonization Code :
3002.14.0000
UNSPSC :
Proteins
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Ambient
Physical State :
Lyophilized
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
Mouse IgG was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
500 µL
Species of Origin :
Mouse
Tested Applications :
SDS-PAGE
Suggested Applications :
ELISA; FC; IF; Multiplex; Other; WB
Storage :
Store purified Mouse IgG at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Synonyms :
Immunoglobulin G from Mouse, IgG from Mouse, purified polyclonal IgG antibody
Background :
Mouse IgG purified protein (Immunoglobulin G) are antibody molecules. Mouse IgG is composed of four peptide chains — two heavy chains and two light chains. Mouse IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. Mouse IgG typically constitutes 75% of serum immunoglobulins. Mouse IgG molecules are synthesized and secreted by plasma B cells. Ideal as a negative control for Flow Cytometry, Western blotting, immunoprecipitation and immunohistochemistry applications.
Low Endotoxin :
No
PSC Format :
Native Protein
PSC Type :
Protein
Sample Size :
No
Application Text :
ELISA,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,
Category :
Proteins and Peptides
Conjugation Name :
Unconjugated
2026 Price :
239.00 USD
Primary Image Name :
SDS-PAGE
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.