This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Rockland Immunochemicals
product type :
protein
product name :
UBE2D2(UBC4) protein-HIS Epitope
catalog :
009-001-U31
quantity :
50 µg
price :
240.00 USD
product information
Catalog Number :
009-001-U31
Name :
UBE2D2(UBC4) recombinant protein-HIS Epitope - 009-001-U31
Display Name :
UBE2D2(UBC4) protein-HIS Epitope
Application Note :
UBE2D2 Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. UBE2D2 Protein is suitable for use in Western Blot. Expect a band approximately ~17kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Buffer :
See application note.
Concentration Value :
0.1 µg/µL
Conjugation :
(None)
Size :
50 µg
Default Unit :
µg
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is one (1) year from date of opening.
Gene Name :
ube2d2
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Purity and Specificity :
Recombinant full length Xenopus UBE2D2 (UBC4) was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
Species Reactivity :
Human
Storage :
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Synonyms :
UBC4, PUBC1, UBC4/5, UBCH5B, E2(17)KB2, ubiquitin-conjugating enzyme E2D 2
Background :
UBE2D2 (UBC4) Protein or ubiquitin-conjugating enzyme E2D 2 is a member of the E2 ubiquitin-conjugating enzyme family which functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 specifically ubiquitinates E6AP and in vivo inhibition of UBE2D2 can lead to inhibition of E6-stimulated p53 degradation (1). UBE2D2 could conjugate ubiquitin to target proteins in an E6AP-dependent manner (2). UBE2D2 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cancer, Cell Cycle, and Neurobiology research.
General Reference :
1. Rolfe, M.et.al: Reconstitution of p53-ubiquitylation reactions from purified components: the role of human ubiquitin-conjugating enzyme UBC4 and E6-associated protein (E6AP). Proc. Nat. Acad. Sci. 92: 3264-3268, 1995. 2. Jensen, J. P.et.al: Identification of a family of closely related human ubiquitin conjugating enzymes. J. Biol. Chem. 270: 30408-30414, 1995.
Low Endotoxin :
No
PSC Format :
Recombinant Protein
PSC Type :
Protein
Sample Size :
No
Application Text :
Western Blot,
Category :
Proteins and Peptides
Conjugation Name :
Unconjugated
2019 Price :
240.00 USD
NCBI :
NM_001093036
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Info@rockland-inc.com
https://rockland-inc.com
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.