AKT1 Human Recombinant Protein | Rockland Immunochemicals 009-001-P21 product information

product summary

company name :

Rockland Immunochemicals

product type :

protein

product name :

AKT1 Human Recombinant Protein

catalog :

009-001-P21

quantity :

price :

342.00 USD

more info or order :

Rockland Immunochemicals product webpage

image

image 1 :

SDS-PAGE of AKT1 Human Recombinant Protein. Lane 1: AKT1, unreduced. Lane 2: prestained MW markers. Lane 3: AKT1, reduced. Load: 1 µg per lane. Predicted/Observed size: 56 kDa, ~56 kDa for AKT1. Other band(s): none.

product information

Catalog Number :

009-001-P21

Name :

AKT1 Human Recombinant Protein - 009-001-P21

Display Name :

AKT1 Human Recombinant Protein

Application Note :

Human AKT1 recombinant protein has been tested in SDS-Page and western blot and is suitable as a control for polyclonal or monoclonal anti-AKT1 in immunological assays. Akt1 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Buffer :

20 mM Tris pH8, 300 mM NaCl with 10% glycerol

Concentration Value :

1.16mg/ml

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

Default Unit :

µg

Western Blot Dilution :

50ng

Expiration :

Expiration date is one (1) year from date of receipt.

Gene Name :

AKT1

Harmonization Code :

3002.14.0000

UNSPSC :

Proteins

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Packing Type :

Dry Ice

Physical State :

Liquid

Purity and Specificity :

Recombinant protein corresponds to amino acids 1 to 480 of mature human AKT1; Akt isoform 1. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.

Species of Origin :

Human

Tested Applications :

SDS-PAGE; WB

Stabilizer :

10% (v/v) Glycerol

Storage :

Store vial at -20°C or colder prior to use. Avoid cycles of freezing and thawing, aliquot and freeze at -20° C or colder. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) .

Synonyms :

RAC, PKB, AKT, PKB alpha, PRKBA, activated AKT

Background :

AKT1 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and RAC (Related to A and C kinases). AKT is a key regulator of many signal transduction pathways, and it exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT1 recombinant protein is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

Low Endotoxin :

PSC Format :

Recombinant Protein

Sample Size :

Application Text :

Western Blot,

Other :

User Optimized

Category :

Proteins and Peptides

Conjugation Name :

Unconjugated

2026 Price :

342.00 USD

UniProt :

P31749

NCBI :

62241011

Primary Image Name :

SDS-PAGE

more info or order :

Rockland Immunochemicals product webpage