This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Rockland Immunochemicals
product type :
protein
product name :
TNF-α Human Recombinant Protein
catalog :
009-001-306
quantity :
50 µg
price :
240.00 USD
product information
Catalog Number :
009-001-306
Name :
TNF-α Human Recombinant Protein - 009-001-306
Display Name :
TNF-α Human Recombinant Protein
Application Note :
TNFα is suitable as a control for polyclonal or monoclonal anti-TNFα in immunological assays.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Conjugation :
(None)
Size :
50 µg
Default Unit :
µg
Expiration :
Expiration date is six (6) months from date of opening.
Gene Name :
TNFA
Host Animal :
Other - E.coli
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data :
Biological Activity: TNF-α activity is determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D and is typically less than 0.05 ng/mL. Endotoxin Level: Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.
Packing Type :
Ambient
Physical State :
Lyophilized
Purity and Specificity :
Purity was determined to be greater than 98% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.
Reconstitution Buffer :
Restore with deionized water (or equivalent)
Reconstitution Volume :
500 µL
Species of Origin :
Human
Species Reactivity :
Human
Storage :
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Synonyms :
Tumor necrosis factor, TNF-alpha, TNF-a, TNFα, cytokine, Tumor necrosis factor ligand superfamily member 2, Cachectin
Background :
TNFα is secreted by macrophages, monocytes, neutrophils, T-cells, and NK- cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha, while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, and GM-CSF. Recombinant Human Tumor Necrosis Factor-alpha produced in E. coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17483.77 Daltons.
Low Endotoxin :
Yes
PSC Format :
Recombinant Protein
PSC Type :
Cytokine, Interleukin or Growth Factor
Sample Size :
No
Other :
User Optimized
Category :
Proteins and Peptides
Conjugation Name :
Unconjugated
2019 Price :
240.00 USD
UniProt :
P01375
Primary Image Name :
TNFa Human Cytokine - SDS-PAGE
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Info@rockland-inc.com
https://rockland-inc.com
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.