product summary
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company name :
Rockland Immunochemicals
product type :
other
product name :
Bovine IgM
catalog :
001-0107
quantity :
1 mg
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
001-0107
Name :
BOVINE IgM whole molecule - 001-0107
Display Name :
Bovine IgM
Application Note :
Bovine IgM whole molecule can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Buffer :
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Concentration Value :
1.0 mg/mL
Concentration Definition :
by UV absorbance at 280 nm
Conjugation :
(None)
Size :
1 mg
Default Unit :
mg
ELISA Dilution :
User Optimized
Immunohistochemistry Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is one (1) year from date of opening.
Format :
IgM
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Wet Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.1% (w/v) Sodium Azide
Purity and Specificity :
Bovine IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.  Bovine IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum and anti-Bovine IgM (µ chain specific).   No reaction was observed against anti-Bovine IgG F(c).  Some light chain cross reactivity will occur with anti-Bovine IgG.
Species of Origin :
Bovine
Storage :
Store vial at 4° C prior to opening.   Bovine IgM whole molecule is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Synonyms :
Cow immunoglobulin M. IgM whole molecule, Bovine IgM
Background :
Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approixmate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Bovine IgM whole molecule is ideal for investigators in Immunology, Microbiology, and Cell Biology.
Low Endotoxin :
No
PSC Format :
Native Protein
PSC Type :
Protein
Sample Size :
No
Application Text :
ELISA,Immunohistochemistry,Western Blot,
Other :
User Optimized
Category :
Proteins and Peptides
Conjugation Name :
Unconjugated
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.