product summary
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company name :
Rockland Immunochemicals
product type :
protein
product name :
Green Fluorescent Protein (GFP) Control Protein
catalog :
000-001-215
quantity :
100 µg
more info or order :
citations: 1
product information
Catalog Number :
000-001-215
Name :
Recombinant Green Fluorescent Protein (GFP) Control Protein - 000-001-215
Display Name :
Green Fluorescent Protein (GFP) Control Protein
Application Note :
GFP Control is suitable as a control for polyclonal or monoclonal Anti-GFP in immunological assays. Researchers should determine optimal titers for applications. The molecular weight of GFP is 28 kDa.
Buffer :
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration Value :
1.0mg/mL
Concentration Definition :
by BCA assay
Conjugation :
(None)
Size :
100 µg
Default Unit :
µg
ELISA Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Dry Ice
Physical State :
Liquid (sterile filtered)
Preservative :
0.01% (w/v) Sodium Azide
Purity and Specificity :
GFP control protein was expressed in E. coli and purified by sequential hydrophobic interaction chromatography, DEAE chromatography and size-exclusion chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-GFP. Analysis by SDS-PAGE resulted in a pattern consistent with purified GFP and was estimated to be greater than 90% pure.
Storage :
Store GFP at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Synonyms :
control protein, rGFP protein, GFP fusion protein, GFP control
Background :
Green Fluorescent Protein (GFP) is used as a positive control when detecting GFP fusion proteins in Anti-GFP antibody assays. Rockland offers multiple purified hosts and conjugated Anti-GFP that can detect GFP by ELISA (sandwich or capture) or western blot. Biotin conjugated polyclonal Anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution when used in combination with monoclonal Anti-GFP (p/n 600-301-215), using either form of the antibody as the capture or detection antibody.
Epitope Tag Type :
GFP
Low Endotoxin :
No
Sample Size :
No
Application Text :
ELISA,Western Blot,
Other :
User Optimized
Category :
GFP and Epitopes
Conjugation Name :
Unconjugated
Primary Image Name :
Anti-GFP Chemiluminescent Kit for Western blot
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.