product summary
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company name :
Rockland Immunochemicals
product type :
protein
product name :
DYKDDDDK (FLAG®) Peptide
catalog :
000-000-383
quantity :
1.0 mg
more info or order :
Rockland Immunochemicals product webpage
product information
Catalog Number :
000-000-383
Name :
DYKDDDDK (FLAG®) Peptide - 000-000-383
Display Name :
DYKDDDDK (FLAG®) Peptide
Application Note :
A working concentration of 500 μg/mL for triple FLAG® peptide is typically used for competitive elution of FLAG® containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG® affinity gel PROTOCOL for specific instructions for use.
Concentration Value :
1 mg/mL
Concentration Definition :
by dry weight
Conjugation :
(None)
Size :
1.0 mg
Default Unit :
mg
Immunoprecipitation Dilution :
YES - see PROTOCOL
Western Blot Dilution :
YES as blocking peptide in PCA
Expiration :
Expiration date is six (6) months from date of opening.
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Ambient
Physical State :
Lyophilized
Purity and Specificity :
Greater than 90% specific peptide by HPLC and MS.
Reconstitution Buffer :
0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4
Reconstitution Volume :
1.0 mL
Storage :
Store vial at 2 - 8 ° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Synonyms :
synthetic FLAG® peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide, blocking peptide, DDK, FLAG
Background :
The FLAG® peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys or DYKDDDDK) is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free FLAG® Peptide.
Epitope Tag Type :
FLAG
Low Endotoxin :
No
PSC Format :
Peptide
PSC Type :
Synthetic Peptide
Sample Size :
No
Application Text :
Immunoprecipitation,Western Blot,
Category :
Proteins and Peptides
Conjugation Name :
Unconjugated
Primary Image Name :
Anti-DYKDDDDK (FLAG® tag) - SDS-PAGE of Simple Purification
more info or order :
Rockland Immunochemicals product webpage
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/
484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.