product summary
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company name :
Rockland Immunochemicals
product type :
other
product name :
TrueBlot® Anti-Rabbit IgG Magnetic Beads
catalog :
00-1800-50
quantity :
5.0 mL
more info or order :
product information
Catalog Number :
00-1800-50
Name :
TrueBlot® Anti-Rabbit IgG Magnetic Beads - 00-1800-50
Display Name :
TrueBlot® Anti-Rabbit IgG Magnetic Beads
Application Note :
TrueBlot® goat Anti-rabbit IgG magnetic beads can be used for separation and purification of rabbit antibodies from serum or rabbit antibody-labeled components, as well as for immunoassays, immunoprecipitation, and IP Western blots. For antibody purification, goat Anti-rabbit IgG magnetic beads are incubated with the rabbit antibody solution and then separated by magnets. After the unbound particulates are washed from the beads, the bound antibodies are eluted from the beads using the elution buffer. The beads are then magnetically separated from the eluted solution, which is removed manually. For IP, target specific antibody is incubated with goat Anti-rabbit IgG magnetic beads. The unbound antibody is washed and the sample containing target antigen is added. After unbound particulates are washed from the beads, the purified protein is eluted from the beads using elution buffer. The samples are then resolved by SDS-PAGE and analyzed by Western blotting.
Buffer :
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality :
Polyclonal
Concentration Value :
5.0 mg/ml
Conjugation :
MAG
Size :
5.0 mL
Default Unit :
mL
Immunoprecipitation Dilution :
User Optimized
Western Blot Dilution :
User Optimized
Expiration :
Expiration date is six (6) months from date of opening.
Format :
IgG
Host Animal :
Goat
General Disclaimer Note :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Packing Type :
Wet Ice
Physical State :
Liquid
Preservative :
0.01% (w/v) Sodium Azide
Species Reactivity :
Rabbit
Storage :
Store vial at 4 °C prior to opening. DO NOT FREEZE.
Synonyms :
Anti-Rabbit Immunoglobulin Gamma, Magnetic beads, nanoparticles, paramagnetic, Magnetic bead-conjugated IgG, Gt-a-Rb IgG, Goat-anti-Rabbit IgG, immunoprecipitation magnetic beads, IP beads, Anti-Rabbit IgG Magnetic beads
Target Species :
Rabbit
Background :
TrueBlot® Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The super-paramagnetic nanoparticles are coupled with a biomolecule, such as goat Anti-rabbit IgG, and are specifically designed, tested and quality controlled for isolation and purification of rabbit IgG, and immunoprecipitation methods using manual or automatic platforms. This antibody binds the heavy chain of all rabbit IgG subclasses and is suitable for immunoassays that utilize a rabbit IgG primary polyclonal antibody. Cell separation and sorting can be achieved using a rabbit IgG antibody to defined cell surface antigens. The beads have a large surface area with high capture efficiencies. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume. Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL.
Low Endotoxin :
No
Sample Size :
No
Application Text :
Immunoprecipitation,Western Blot,
Other :
User Optimized
Category :
Avidin, Biotin, Streptavidin
Conjugation Name :
Magnetic Bead
more info or order :
company information
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
tech@rockland.com
https://www.rockland.com/484-791-3823
headquarters: USA
Rockland Immunochemicals, Inc. produces Phosho-Site Specific Antibodies and Antibody based tools for basic, applied and clinical research. Our laboratory is located west of Philadelphia, Pennsylvania, USA. The technology base of the organization is the experience of its staff scientists in the production and purification of a wide range of antibodies through monoclonal & polyclonal antibody techniques utilizing in vitro and in vivo methods. Rockland's antibodies are suited for individuals performing Western Blotting, ELISA, Immunohistochemistry, Fluorescent Microscopy, High Content Screening and diagnostic kit production. Primary antibodies include Akt Pathway, Apolipoproteins, Apoptosis/Cell Cycle, Cytokines, Cell Signaling, Enzymes, Extracellular Matrix, Transcription Factors (NFkB) and Ubiquitin.