Recombinant Human/Mouse/Rat NEDD8 Protein, CF | R&D Systems UL-812-500 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human/Mouse/Rat NEDD8 Protein, CF

catalog :

UL-812-500

quantity :

500 ug

price :

246 USD

more info or order :

R&D Systems product webpage

citations: 8

Reference
Wu K, DeVita R, Pan Z. Monoubiquitination empowers ubiquitin chain elongation. J Biol Chem. 2024;300:105753 pubmed publisher
Banchenko S, Krupp F, Gotthold C, Bürger J, Graziadei A, O Reilly F, et al. Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses. PLoS Pathog. 2021;17:e1009775 pubmed publisher
Cathcart A, Bird G, Wales T, Herce H, Harvey E, Hauseman Z, et al. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer. Nat Chem Biol. 2020;16:1218-1226 pubmed publisher
Liu X, Reitsma J, Mamrosh J, Zhang Y, Straube R, Deshaies R. Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression. Mol Cell. 2018;69:773-786.e6 pubmed publisher
Zhou H, Lu J, Liu L, Bernard D, Yang C, Fernandez Salas E, et al. A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation. Nat Commun. 2017;8:1150 pubmed publisher
Huang G, Towe C, Choi L, Yonekawa Y, Bommelje C, Bains S, et al. The ubiquitin-associated (UBA) domain of SCCRO/DCUN1D1 protein serves as a feedback regulator of biochemical and oncogenic activity. J Biol Chem. 2015;290:296-309 pubmed publisher
Huang G, Stock C, Bommelje C, Weeda V, Shah K, Bains S, et al. SCCRO3 (DCUN1D3) antagonizes the neddylation and oncogenic activity of SCCRO (DCUN1D1). J Biol Chem. 2014;289:34728-42 pubmed publisher
Stanley D, Bartholomeeusen K, Crosby D, Kim D, Kwon E, Yen L, et al. Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G. PLoS Pathog. 2012;8:e1003085 pubmed publisher

product information

master code :

UL-812

SKU :

UL-812-500

product name :

Recombinant Human/Mouse/Rat NEDD8 Protein, CF

unit size :

500 ug

description :

The Recombinant Human/Mouse/Rat NEDD8 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human/Mouse/Rat NEDD8 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

NEDD8

category :

Proteins and Enzymes

buffer :

Supplied as a solution in HEPES, NaCl and DTT.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain.

species :

Human, Mouse, Rat

theoretical molecular weight :

8.6 kDa

gene symbol :

NEDD8

details of functionality :

Recombinant Human NEDD8 can be conjugated to substrate proteins via the subsequent actions of a NEDD8-activating (E1) enzyme, a NEDD8-conjugating (E2) enzyme, and a NEDD8 ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human NEDD8 concentration of 10-50 ╡M.

accessionNumbers :

Q15843.1

applications :

Enzyme Activity

source long :

E. coli -derived NEDD8 protein Met1 - Gly76

source short :

E. coli

USD :

246 USD

alt names :

FLJ43224, MGC104393, MGC125896, MGC125897, NEDD-8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, neural precursor cell expressed, developmentally down-regulated 8, Rub1, Ubiquitin-like protein Nedd8

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage