Recombinant Human ISG15 AMC Protein, CF | R&D Systems UL-553 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human ISG15 AMC Protein, CF

catalog :

UL-553

quantity :

50 ug

price :

285 USD

citations: 8

Reference
Paudel P, Zhang Q, Leung C, Greenberg H, Guo Y, Chern Y, et al. Crystal structure and activity-based labeling reveal the mechanisms for linkage-specific substrate recognition by deubiquitinase USP9X. Proc Natl Acad Sci U S A. 2019;116:7288-7297 pubmed publisher
Hermanns T, Pichlo C, Woiwode I, Klopffleisch K, Witting K, Ovaa H, et al. A family of unconventional deubiquitinases with modular chain specificity determinants. Nat Commun. 2018;9:799 pubmed publisher
Daczkowski C, Dzimianski J, Clasman J, Goodwin O, Mesecar A, Pegan S. Structural Insights into the Interaction of Coronavirus Papain-Like Proteases and Interferon-Stimulated Gene Product 15 from Different Species. J Mol Biol. 2017;429:1661-1683 pubmed publisher
Beckmann J, Ronau J, Hochstrasser M. A Wolbachia deubiquitylating enzyme induces cytoplasmic incompatibility. Nat Microbiol. 2017;2:17007 pubmed publisher
Clasman J, Baez Santos Y, Mettelman R, O Brien A, Baker S, Mesecar A. X-ray Structure and Enzymatic Activity Profile of a Core Papain-like Protease of MERS Coronavirus with utility for structure-based drug design. Sci Rep. 2017;7:40292 pubmed publisher
Chen Y, Savinov S, Mielech A, Cao T, Baker S, Mesecar A. X-ray Structural and Functional Studies of the Three Tandemly Linked Domains of Non-structural Protein 3 (nsp3) from Murine Hepatitis Virus Reveal Conserved Functions. J Biol Chem. 2015;290:25293-306 pubmed publisher
Deaton M, Spear A, Faaberg K, Pegan S. The vOTU domain of highly-pathogenic porcine reproductive and respiratory syndrome virus displays a differential substrate preference. Virology. 2014;454-455:247-53 pubmed publisher
Basters A, Ketscher L, Deuerling E, Arkona C, Rademann J, Knobeloch K, et al. High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system. BMC Biotechnol. 2012;12:56 pubmed publisher

product information

brand :

R&D Systems, powered by Boston Biochem

master code :

UL-553

SKU :

UL-553-050

product name :

Recombinant Human ISG15 AMC Protein, CF

description :

The Recombinant Human ISG15 AMC Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human ISG15 AMC Protein, CF has been validated for the following applications: Enzyme Activity.

target :

ISG15 / UCRP

category :

Proteins and Enzymes

unit size :

50 ug

buffer :

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 20% Glycerol (v/v), 2mM DTT

conjugate :

7-Amino-4-Methyl Coumarin

main entrez gene id :

9636

species :

Human

theoretical molecular weight :

19 kDa

gene symbol :

ISG15

details of functionality :

Recombinant Human ISG15 AMC is a fluorogenic substrate for some ISG15-specific isopeptidases. Release of AMC fluorescence can be monitored with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human ISG15 AMC concentration of 0.1-1 µM.

catalog number base :

UL-553

accessionNumbers :

P05161

applications :

Enzyme Activity

source :

USD :

285 USD

alt names :

G1P2, G1P2interferon-induced 17-kDa/15-kDa protein, IFI15, IFI15hUCRP, interferon, alpha-inducible protein (clone IFI-15K), Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, interferon-stimulated protein, 15 kDa, IP17, ISG15 ubiquitin-like modifier, Ubiquitin cross-reactive protein, ubiquitin-like protein ISG15, UCRP, UCRPIP17

storage :

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.