Recombinant Human ISG15 AMC Protein, CF | R&D Systems UL-553-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human ISG15 AMC Protein, CF

catalog :

UL-553-050

quantity :

50 ug

price :

410 USD

more info or order :

R&D Systems product webpage

citations: 12

Reference
Bonacci T, Bolhuis D, Brown N, Emanuele M. Mechanisms of USP18 deISGylation revealed by comparative analysis with its human paralog USP41. bioRxiv. 2024;: pubmed publisher
van Huizen M, Bloeme Ter Horst J, De Gruyter H, Geurink P, van der Heden van Noort G, Knaap R, et al. Deubiquitinating activity of SARS-CoV-2 papain-like protease does not influence virus replication or innate immune responses in vivo. PLoS Pathog. 2024;20:e1012100 pubmed publisher
Patchett S, Lv Z, Rut W, Bekes M, Drag M, Olsen S, et al. A molecular sensor determines the ubiquitin substrate specificity of SARS-CoV-2 papain-like protease. Cell Rep. 2021;36:109754 pubmed publisher
Xia Z, Sacco M, Ma C, Townsend J, Kitamura N, Hu Y, et al. Discovery of SARS-CoV-2 papain-like protease inhibitors through a combination of high-throughput screening and FlipGFP-based reporter assay. bioRxiv. 2021;: pubmed publisher
Paudel P, Zhang Q, Leung C, Greenberg H, Guo Y, Chern Y, et al. Crystal structure and activity-based labeling reveal the mechanisms for linkage-specific substrate recognition by deubiquitinase USP9X. Proc Natl Acad Sci U S A. 2019;116:7288-7297 pubmed publisher
Hermanns T, Pichlo C, Woiwode I, Klopffleisch K, Witting K, Ovaa H, et al. A family of unconventional deubiquitinases with modular chain specificity determinants. Nat Commun. 2018;9:799 pubmed publisher
Daczkowski C, Dzimianski J, Clasman J, Goodwin O, Mesecar A, Pegan S. Structural Insights into the Interaction of Coronavirus Papain-Like Proteases and Interferon-Stimulated Gene Product 15 from Different Species. J Mol Biol. 2017;429:1661-1683 pubmed publisher
Beckmann J, Ronau J, Hochstrasser M. A Wolbachia deubiquitylating enzyme induces cytoplasmic incompatibility. Nat Microbiol. 2017;2:17007 pubmed publisher
Clasman J, Baez Santos Y, Mettelman R, O Brien A, Baker S, Mesecar A. X-ray Structure and Enzymatic Activity Profile of a Core Papain-like Protease of MERS Coronavirus with utility for structure-based drug design. Sci Rep. 2017;7:40292 pubmed publisher
Chen Y, Savinov S, Mielech A, Cao T, Baker S, Mesecar A. X-ray Structural and Functional Studies of the Three Tandemly Linked Domains of Non-structural Protein 3 (nsp3) from Murine Hepatitis Virus Reveal Conserved Functions. J Biol Chem. 2015;290:25293-306 pubmed publisher
Deaton M, Spear A, Faaberg K, Pegan S. The vOTU domain of highly-pathogenic porcine reproductive and respiratory syndrome virus displays a differential substrate preference. Virology. 2014;454-455:247-53 pubmed publisher
Basters A, Ketscher L, Deuerling E, Arkona C, Rademann J, Knobeloch K, et al. High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system. BMC Biotechnol. 2012;12:56 pubmed publisher

product information

master code :

UL-553

SKU :

UL-553-050

product name :

Recombinant Human ISG15 AMC Protein, CF

unit size :

50 ug

description :

The Recombinant Human ISG15 AMC Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human ISG15 AMC Protein, CF has been validated for the following applications: Enzyme Activity.

target :

ISG15/UCRP

category :

Proteins and Enzymes

buffer :

Supplied as a solution in HEPES, NaCl, Glycerol and DTT.

conjugate :

7-Amino-4-Methyl Coumarin

species :

Human

theoretical molecular weight :

18 kDa

gene symbol :

ISG15

details of functionality :

This fluorogenic substrate for ISG15 hydrolases is based on the carboxy-terminus derivatization of ISG15 with 7-amido-4-methylcoumarin (AMC). Release of AMC fluorescence can be monitored with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human ISG15 AMC concentration of 0.1-1 ╡M.

accessionNumbers :

P05161.5

applications :

Enzyme Activity

source long :

E. coli -derived human ISG15/UCRP protein Met1 - Cys157 (C78S, G157C) with a C-terminal 6-His tag; C-terminal AMC derivatized protein

source short :

E. coli

USD :

410 USD

alt names :

G1P2, G1P2interferon-induced 17-kDa/15-kDa protein, IFI15, IFI15hUCRP, interferon, alpha-inducible protein (clone IFI-15K), Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, interferon-stimulated protein, 15 kDa, IP17, ISG15 ubiquitin-like modifier, Ubiquitin cross-reactive protein, ubiquitin-like protein ISG15, UCRP, UCRPIP17

storage :

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage