Recombinant Human NEDD8 AMC Protein, CF | R&D Systems UL-552 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human NEDD8 AMC Protein, CF

catalog :

UL-552

quantity :

50 ug

price :

275 USD

citations: 5

Reference
Karpiyevich M, Adjalley S, Mol M, Ascher D, Mason B, van der Heden van Noort G, et al. Nedd8 hydrolysis by UCH proteases in Plasmodium parasites. PLoS Pathog. 2019;15:e1008086 pubmed publisher
Paudel P, Zhang Q, Leung C, Greenberg H, Guo Y, Chern Y, et al. Crystal structure and activity-based labeling reveal the mechanisms for linkage-specific substrate recognition by deubiquitinase USP9X. Proc Natl Acad Sci U S A. 2019;116:7288-7297 pubmed publisher
Beckmann J, Ronau J, Hochstrasser M. A Wolbachia deubiquitylating enzyme induces cytoplasmic incompatibility. Nat Microbiol. 2017;2:17007 pubmed publisher
Urbanus M, Quaile A, Stogios P, Morar M, Rao C, Di Leo R, et al. Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen, Legionella pneumophila. Mol Syst Biol. 2016;12:893 pubmed publisher
Birol M, Enchev R, Padilla A, Stengel F, Aebersold R, Betzi S, et al. Structural and biochemical characterization of the Cop9 signalosome CSN5/CSN6 heterodimer. PLoS ONE. 2014;9:e105688 pubmed publisher

product information

brand :

R&D Systems, powered by Boston Biochem

master code :

UL-552

SKU :

UL-552-050

product name :

Recombinant Human NEDD8 AMC Protein, CF

description :

The Recombinant Human NEDD8 AMC Protein, CF from R&D Systems, powered by Boston Biochem is derived from E. coli. The Recombinant Human NEDD8 AMC Protein, CF has been validated for the following applications: Enzyme Activity.

target :

NEDD8

category :

Proteins and Enzymes

unit size :

50 ug

buffer :

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl

conjugate :

7-Amino-4-Methyl Coumarin

main entrez gene id :

4738

purity :

>95%, by HPLC.

species :

Human

theoretical molecular weight :

8.7 kDa

gene symbol :

NEDD8

details of functionality :

Recombinant Human NEDD8 AMC is a fluorogenic substrate for some NEDD8-specific isopeptidases. Release of AMC fluorescence can be monitored with an excitation wavelength of 345 nm and an emission wavelength of 445 nm. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human NEDD8 AMC concentration of 0.1-1 µM.

catalog number base :

UL-552

accessionNumbers :

Q15843

applications :

Enzyme Activity

source :

USD :

275 USD

alt names :

FLJ43224, MGC104393, MGC125896, MGC125897, NEDD-8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, neural precursor cell expressed, developmentally down-regulated 8, Rub1, Ubiquitin-like protein Nedd8

storage :

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.