AQUApure Di-Ub Chains (K6-linked) Protein, CF | R&D Systems UC-11B-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

AQUApure Di-Ub Chains (K6-linked) Protein, CF

catalog :

UC-11B-025

quantity :

25 ug

more info or order :

R&D Systems product webpage

citations: 9

Reference
Shi Y, Liu H, Ma K, Luo Z, Qiu J. Legionella longbeachae effector protein RavZ inhibits autophagy and regulates phagosome ubiquitination during infection. PLoS ONE. 2023;18:e0281587 pubmed publisher
Manczyk N, Veggiani G, Teyra J, Strilchuk A, Sidhu S, Sicheri F. The ubiquitin interacting motifs of USP37 act on the proximal Ub of a di-Ub chain to enhance catalytic efficiency. Sci Rep. 2019;9:4119 pubmed publisher
Haahr P, Borgermann N, Guo X, Typas D, Achuthankutty D, Hoffmann S, et al. ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability. Mol Cell. 2018;70:165-174.e6 pubmed publisher
Rasool S, Soya N, Truong L, Croteau N, Lukacs G, Trempe J. PINK1 autophosphorylation is required for ubiquitin recognition. EMBO Rep. 2018;19: pubmed publisher
Zhao Y, Mudge M, Soll J, Rodrigues R, Byrum A, Schwarzkopf E, et al. OTUD4 Is a Phospho-Activated K63 Deubiquitinase that Regulates MyD88-Dependent Signaling. Mol Cell. 2018;69:505-516.e5 pubmed publisher
Zheng H, Wang M, Zhao C, Wu S, Yu P, Lu Y, et al. Characterization of the deubiquitination activity and substrate specificity of the chicken ubiquitin-specific protease 1/USP associated factor 1 complex. PLoS ONE. 2017;12:e0186535 pubmed publisher
Wang Y, Bian Y, Luo J, Lu M, Xiong Y, Guo S, et al. Cholesterol and fatty acids regulate cysteine ubiquitylation of ACAT2 through competitive oxidation. Nat Cell Biol. 2017;19:808-819 pubmed publisher
Tsuchiya H, Ohtake F, Arai N, Kaiho A, Yasuda S, Tanaka K, et al. In Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-Linked Chain Specificity of the Proteasome. Mol Cell. 2017;66:488-502.e7 pubmed publisher
He F, Wollscheid H, Nowicka U, Biancospino M, Valentini E, Ehlinger A, et al. Myosin VI Contains a Compact Structural Motif that Binds to Ubiquitin Chains. Cell Rep. 2016;14:2683-94 pubmed publisher

product information

master code :

UC-11B

SKU :

UC-11B-025

product name :

AQUApure Di-Ub Chains (K6-linked) Protein, CF

unit size :

25 ug

description :

The AQUApure Di-Ub Chains (K6-linked) Protein, CF from R&D Systems is derived from E. coli. The AQUApure Di-Ub Chains (K6-linked) Protein, CF has been validated for the following applications: Bioactivity.

target :

Di-Ubiquitin

category :

Proteins and Enzymes

buffer :

1 mg/ml (58 ╡M) in sterile, deionized water

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain. . Ub-AQUA analysis: . K6 : 99.19% . K63: 0.40% . K48: 0.22% . K11: 0.14% . All other linkages .

species :

Multi-Species

theoretical molecular weight :

17 kDa

details of functionality :

Ubiquitin chains vary in length, linkage, and function. K6-linked Di-Ubiquitin Chains (Ub2) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at 40 ░C for 20 minutes prior to gel electrophoresis.

accessionNumbers :

P0CG47

applications :

Bioactivity

source long :

E. coli -derived Di-Ubiquitin protein

source short :

E. coli

extended description :

Will be discontinued when existing inventory is gone.

product details :

Will be discontinued when existing inventory is gone.

alt names :

DiUbiquitin, HEL-S-50, Ub2, UBB, ubiquitin B

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage