Recombinant Human Ubiquitin N-Terminal Biotin Protein, CF | R&D Systems UB-560-050 product information

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product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Ubiquitin N-Terminal Biotin Protein, CF

catalog :

UB-560-050

quantity :

50 ug

price :

294 USD

citations: 5

Reference
Fiesel F, Fri x10d ov xe1 D, Hayes C, Coban M, Hudec R, Bredenberg J, et al. Substitution of PINK1 Gly411 modulates substrate receptivity and turnover. Autophagy. 2023;19:1711-1732 pubmed publisher
Hou X, Fiesel F, Truban D, Castanedes Casey M, Lin W, Soto A, et al. Age- and disease-dependent increase of the mitophagy marker phospho-ubiquitin in normal aging and Lewy body disease. Autophagy. 2018;14:1404-1418 pubmed publisher
Ando M, Fiesel F, Hudec R, Caulfield T, Ogaki K, GÃ³rka Skoczylas P, et al. The PINK1 p.I368N mutation affects protein stability and ubiquitin kinase activity. Mol Neurodegener. 2017;12:32 pubmed publisher
Puschmann A, Fiesel F, Caulfield T, Hudec R, Ando M, Truban D, et al. Heterozygous PINK1 p.G411S increases risk of Parkinson's disease via a dominant-negative mechanism. Brain. 2017;140:98-117 pubmed publisher
Smit J, Monteferrario D, Noordermeer S, van Dijk W, van der Reijden B, Sixma T. The E3 ligase HOIP specifies linear ubiquitin chain assembly through its RING-IBR-RING domain and the unique LDD extension. EMBO J. 2012;31:3833-44 pubmed publisher

product information

master code :

UB-560

SKU :

UB-560-050

product name :

Recombinant Human Ubiquitin N-Terminal Biotin Protein, CF

unit size :

50 ug

description :

The Recombinant Human Ubiquitin N-Terminal Biotin Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human Ubiquitin N-Terminal Biotin Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Ubiquitin

category :

Proteins and Enzymes

buffer :

Supplied as a solution in 10 mM HEPES pH 8.0

conjugate :

Biotin

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain.

species :

Human

theoretical molecular weight :

9 kDa

gene symbol :

UBB

details of functionality :

Biotinylated Recombinant Human Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend using Biotinylated Recombinant Human Ubiquitin in conjunction with native Ubiquitin at a combined concentration of 10-50 ╡M with a 1:2 to 1:20 ratio of Biotinylated Recombinant Human Ubiquitin:native Ubiquitin. The resulting poly-Ubiquitin chains can be visualized/quantitated with avidin-linked detection reagents.

accessionNumbers :

P0CG47.1

applications :

Enzyme Activity

source long :

E. coli -derived human Ubiquitin protein Gln2-Gly76 with a single N-terminal biotin

source short :

E. coli

USD :

294 USD

alt names :

RPS27A, UBA52, UBB, UBB ubiquitin B, UBC

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.